Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens.
Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens.
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DOI：
10.1084/jem.144.4.873
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发表时间：
1976-10-01
期刊：
影响因子：
--
通讯作者：
Oettgen HF
中科院分区：
文献类型：
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作者：
Shiku H;Takahashi T;Oettgen HF
免疫粘附测定显示，18 名黑色素瘤患者中有 10 名具有明显的针对自体培养黑色素瘤细胞表面抗原的抗体，血清滴度范围为 1/4 至 1/160。自体成纤维细胞与这些血清没有反应。来自个体患者的抗体表现出对最大反应性的可重复的温度偏好。本研究定义了两种新的黑色素瘤抗原系统。第一个，BD，仅限于自体黑色素瘤，无法在 12 种同种异体黑色素瘤细胞系的吸收测试中得到证实。另一种是 AH，在 12 种黑色素瘤中的 5 种中发现，代表一类共有的黑色素瘤表面抗原。在自体、同种异体或异种来源的正常细胞或任何非黑色素瘤肿瘤细胞系上均未发现 BD 和 AH 抗原。现在可用于开发黑色素瘤表面抗原的全面血清学分类的方法。
Immune adherence assays revealed that 10 out of 18 melanoma patients had demonstrable antibody to surface antigens of autologous cultured melanoma cells, with serum titers ranging from 1/4 to 1/160. Autologous fibroblasts showed no reactions with these sera. Antibody from individual patients showed reproducible temperature preference for maximal reactivity. Two new melanoma antigenic systems were defined in this study. The first, BD, was restricted to autologous melanoma and could not be demonstrated in absorption tests on 12 allogeneic melanoma cell lines. The other, AH, was found on 5 of 12 melanomas and represents a class of shared melanoma surface antigens. Neither BD nor AH antigen was found on normal cells from autologous, allogeneic, or xenogeneic sources or on any nonmelanoma tumor cell line. Methods are now available to develop a comprehensive serological classification of the surface antigens of melanoma.