Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate
Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate
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DOI：
10.1007/s10529-006-9204-6
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Hao, Jianrong;Ma, Cuiqing;Xu, Ping
施氏假单胞菌SDM在24小时内将DL-乳酸（25.5 g l（-1））氧化为甘草酸（22.6 g l（-1））。基于酶活性测定和蛋白质组学分析，首次在乳酸生物转化为丙酮酸的过程中发现了NAD（+）非依赖性D-乳酸脱氢酶和NAD+非依赖性L-乳酸脱氢酶。
Pseudomonas stutzeri SDM oxidized DL-lactic acid (25.5 g l(-1)) into pyruvic acid (22.6 g l(-1)) over 24 h. Both NAD(+)- independent D-lactate dehydrogenase and NAD+- independent L-lactate dehydrogenase were found for the first time in the bioconversion of lactate to pyruvate based on the enzyme activity assay and proteomic analysis.