Morphological signs of apoptosis in axotomized ganglion cells of the rabbit retina
Morphological signs of apoptosis in axotomized ganglion cells of the rabbit retina
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DOI：
10.1016/j.neuroscience.2006.10.039
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发表时间：
2007-02-09
期刊：
影响因子：
3.3
通讯作者：
La Villa, P. De
中科院分区：
文献类型：
--
作者：
Germain, F.;Fernandez, E.;La Villa, P. De
哺乳动物视神经切片可诱导视网膜神经节细胞(RGC)的凋亡性死亡。然而，少数RGC存活的时间相对较长。这些细胞在凋亡过程中经历了显著的形态变化，但其中一些变化与坏死细胞中的变化没有明显的区别。本工作对兔视神经切断1个月后注射神经生物素后1个月的视网膜神经节细胞进行了光镜、透射电子显微镜和扫描电子显微镜观察。用末端脱氧核苷酸转移酶生物素-dUTP缺口末端标记法检测细胞凋亡，并在电镜图像中观察到典型的细胞凋亡征象。超微结构分析显示胞浆内空泡变性，正常细胞结构消失。扫描电子显微镜观察到轴突损伤的视网膜节细胞有膜改变的迹象。细胞膜的早期变化表明，轴突切断可能会导致细胞骨架的重要变化。结论：兔视网膜神经节细胞切断轴突后，在细胞膜水平观察到明显的细胞凋亡特征，可能是细胞死亡的原因之一。(C)2006年IBRO。爱思唯尔有限公司出版。保留所有权利。
Optic nerve section in mammals induces apoptotic death of retinal ganglion cells (RGCs). However, a small population of RGCs survives for a relatively long time. These cells experience significant morphological changes due to the apoptotic process, but some of these changes are not clearly differentiated from those experienced in necrotic cells. In the present work, rabbit RGCs were studied 1 month after optic nerve section using light microscopy after neurobiotin injection, transmission electron microscopy (EM) and scanning electron microscopy (SEM). Apoptosis was identified by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling and characteristic signs of apoptosis were observed in the EM images. Ultrastructural analyses showed vacuolar degeneration in the cytoplasm and normal cellular structure loss. Signs of membrane changes were observed in axotornized RGCs by SEM. Early changes seen in the cell membrane suggest that axotomy may cause important changes in the cytoskeleton. We conclude that characteristic signs of apoptosis at the cell membrane level are clearly observed in rabbit RGCs after axotomy and they may be responsible for the cellular death. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.