Knockdown of STEAP1 inhibits cell growth and induces apoptosis in LNCaP prostate cancer cells counteracting the effect of androgens
Knockdown of STEAP1 inhibits cell growth and induces apoptosis in LNCaP prostate cancer cells counteracting the effect of androgens
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DOI：
10.1007/s12032-018-1100-0
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发表时间：
2018-03-01
期刊：
影响因子：
3.4
通讯作者：
Maia, Claudio Jorge
中科院分区：
文献类型：
--
作者：
Gomes, Ines Margarida;Rocha, Sandra Moreira;Maia, Claudio Jorge
前列腺六跨膜上皮抗原1（STEAP 1）在许多类型的肿瘤中过表达，特别是在前列腺癌中。STEAP 1位于上皮细胞的质膜上，可能在细胞间和细胞内通讯中起重要作用。一些研究表明STEAP 1是前列腺癌的潜在生物标志物和免疫靶点。然而，STEAP 1在细胞增殖和凋亡中的作用仍不清楚。因此，STEAP 1在前列腺癌细胞增殖和凋亡中的作用是通过诱导STEAP 1基因在LNCaP细胞中的敲低来确定的。此外，评价了DHT对STEAP 1基因敲低的LNCaP细胞增殖的影响。我们的研究结果表明，沉默STEAP 1基因降低LNCaP细胞活力和增殖，同时诱导凋亡。此外，我们发现STEAP 1基因敲低的细胞和分子效应可能不依赖于DHT治疗，阻断STEAP 1可能是激活癌细胞凋亡的适当策略，也可以阻止DHT在前列腺癌中的增殖和抗凋亡作用。
Six transmembrane epithelial antigen of the prostate 1 (STEAP1) is overexpressed in numerous types of tumors, especially in prostate cancer. STEAP1 is located in the plasma membrane of epithelial cells and may play an important role in inter-and intracellular communication. Several studies suggest STEAP1 as a potential biomarker and an immunotherapeutic target for prostate cancer. However, the role of STEAP1 in cell proliferation and apoptosis remains unclear. Therefore, the role of STEAP1 in prostate cancer cells proliferation and apoptosis was determined by inducing STEAP1 gene knockdown in LNCaP cells. In addition, the effect of DHT on the proliferation of LNCaP cells knocked down for STEAP1 gene was evaluated. Our results demonstrated that silencing the STEAP1 gene reduces LNCaP cell viability and proliferation, while inducing apoptosis. In addition, we showed that the cellular and molecular effects of STEAP1 gene knockdown may be independent of DHT treatment, and blocking STEAP1 may reveal to be an appropriate strategy to activate apoptosis in cancer cells, as well as to prevent the proliferative and anti-apoptotic effects of DHT in prostate cancer.