Evaluating the challenges associated with time-of-fight secondary ion mass spectrometry for metabolomics using pure and mixed metabolites
Evaluating the challenges associated with time-of-fight secondary ion mass spectrometry for metabolomics using pure and mixed metabolites
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DOI：
10.1007/s11306-012-0487-4
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发表时间：
2013-06-01
期刊：
影响因子：
3.6
通讯作者：
Vickerman, John C.
中科院分区：
文献类型：
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作者：
Fletcher, John S.;Kotze, Helen L.;Vickerman, John C.
飞行时间二次离子质谱 (ToF-SIMS) 很有可能有助于代谢组分析，同时带来高分辨率、无标记成像的额外优势。用于从样品中解吸物质的聚焦离子束可以聚焦在 1 μm 以下，从而实现亚细胞尺度的化学成像。在本研究中，我们测试了 ToF-SIMS 从一组标准代谢物生成质谱和 MSMS 谱图的能力，这些谱图可以与包含 ESI-CID MSMS 谱图的开放存取代谢物数据库进行比较。使用混合代谢物样品和在化合物鉴定和定量方面讨论的数据来评估化学环境、基质效应对观察到的质谱的影响。自由基离子和小碎片离子似乎对离子抑制或增强不太敏感，并且可能提供定量的途径。了解这些参数对于 ToF-SIMS 原位代谢组学技术的成功应用至关重要。
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is potentially well placed to contribute to metabolomic analysis while bringing the added benefit of high resolution, label free imaging. The focused ion beams used to desorb species from the sample can be focused below 1 mu m allowing chemical imaging on a sub-cellular scale. In this study we test the capability of ToF-SIMS to generate mass spectrometry and MSMS spectra from a set of standard metabolites that can be compared with open access metabolite databases containing ESI-CID MSMS spectra. The influence of the chemical environment, the matrix effect, on the observed mass spectra is assessed using a mixed metabolite sample and the data discussed in terms of compound identification and quantification. Radical ions and small fragment ions seem to be less sensitive to ion suppression or enhancement and may provide a route to quantification. Understanding such parameters will be key for the successful application of the technique for in situ metabolomics with ToF-SIMS.