Chemical receptors of the arytenoid: A comparison of human and mouse
Chemical receptors of the arytenoid: A comparison of human and mouse
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DOI：
10.1002/lary.27931
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发表时间：
2020-02-01
期刊：
影响因子：
2.6
通讯作者：
Finger, Thomas E.
中科院分区：
文献类型：
--
作者：
Jette, Marie E.;Clary, Matthew S.;Finger, Thomas E.
目的/假设喉部是一个高度敏感的器官，暴露于机械、热和化学刺激下。化学物质在上皮内神经纤维和特殊的化学感觉细胞（包括分散的孤立细胞和组成味蕾的味觉细胞）中引起反应。喉部化学感觉细胞和味蕾的激活，在接触酸或苦的物质，甚至是水或甜味的化学物质时，都会引起咳嗽、吞咽或呼吸暂停。为了开始理解它们的功能，我们试图比较人类和小鼠喉部上皮中化学感觉细胞和化学反应神经纤维的分布、密度和类型。研究设计动物和人类实验室分析。方法：采用免疫组化方法，我们在以下两例患者的喉上皮类蝶突区鉴定了味觉细胞和多模性伤害神经纤维：1)接受喉软化症声门上成形术的婴儿；2)来自34岁供体的尸体标本。然后，我们将这些发现与断奶前和成熟小鼠组织进行了比较。结果人和小鼠的类arytena组织中含有大量的味蕾，这些味蕾含有苦味、甜味和鲜味的II型味觉细胞，这些味蕾由表达P2X3型三磷酸腺苷受体的神经纤维支配。III型细胞（酸反应）也存在，但它们在人体组织中的数量少于小鼠组织中的数量。在这两种中，上皮被自由神经末梢密集支配。结论：从化学感觉的角度来看，人和小鼠的喉部在生物学上是相似的。这表明小鼠模型可以有效地用于喉部化学感觉研究。证据水平喉镜，130:423- 431,2020
Objectives/Hypothesis The larynx is a highly responsive organ exposed to mechanical, thermal, and chemical stimuli. Chemicals elicit responses both in intraepithelial nerve fibers and in specialized chemosensory cells, including scattered solitary cells as well as taste cells organized into taste buds. Activation of both chemosensory cells and taste buds in the larynx elicit cough, swallow, or apnea with exposure to sour or bitter substances, and even by water or sweet-tasting chemicals. In an effort to begin understanding their function, we sought to compare the distribution, density, and types of chemosensory cells and chemoresponsive nerve fibers in laryngeal epithelium of humans and mice. Study Design Animal and human laboratory analysis. Methods Using immunohistochemistry, we identified taste cells and polymodal nociceptive nerve fibers in the arytenoid area of the laryngeal epithelium of the following: 1) infants undergoing supraglottoplasty for laryngomalacia, and 2) a cadaveric specimen procured from a 34-year-old donor. We then compared these findings to both preweanling and mature mouse tissue. Results Arytenoid tissue from both human and mouse contained many taste buds containing type II taste cells-bitter, sweet, or umami sensing-which were innervated by nerve fibers expressing P2X3 type adenosine triphosphate receptors. Type III cells (acid responsive) were also present, but they were fewer in human tissue than in equivalent tissue from mice. In both species, the epithelium was densely innervated by free nerve endings. Conclusions Our findings suggest that from a standpoint of chemosensation, human and mouse larynges are biologically similar. This suggests that a murine model can be used effectively in laryngeal chemosensory research. Level of Evidence NA Laryngoscope, 130:423-430, 2020