Effect of basic fibroblast growth factor on cartilage regeneration in chondrocyte-seeded collagen sponge scaffold
Effect of basic fibroblast growth factor on cartilage regeneration in chondrocyte-seeded collagen sponge scaffold
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DOI：
10.1016/0142-9612(96)85760-7
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发表时间：
1996-01-01
期刊：
影响因子：
14
通讯作者：
Ikada, Y
中科院分区：
文献类型：
--
作者：
Fujisato, T;Sajiki, T;Ikada, Y
制备软骨细胞-胶原复合物，并将其植入裸鼠皮下进行软骨再生。当复合材料在植入前用碱性成纤维细胞生长因子（bFGF）浸渍时，软骨组织的再生显着加速。用番红O-坚牢绿色对植入的复合材料进行组织学染色，显示掺入复合材料中的细胞表现出其表型并形成新的成熟软骨。在植入的复合材料周围观察到一层薄薄的纤维囊，宿主对植入物的炎症反应轻微。植入后1周，复合材料中仍有特异性蛋白多糖积累。在植入后2周，软骨细胞再生了软骨组织，虽然仍然不成熟，但在4周时几乎所有的软骨细胞都转移到成熟阶段。相反，如果胶原支架没有用bFGF浸渍，则在植入后4周内不会注意到这种成熟的软骨组织。此外，成熟区域仅限于植入复合材料的一小部分，除非将bFGF掺入其中。
A chondrocyte-collagen composite was prepared in an attempt to regenerate cartilage by its subcutaneous implantation in nude mouse. When the composite was impregnated with basic fibroblast growth factor (bFGF) prior to implantation, regeneration of the cartilage tissue was remarkably accelerated. Histological staining of the implanted composites with Safranin O-fast green revealed that the cells incorporated in the composites exhibited their phenotype and formed a new matured cartilage. A thin layer of fibrous capsule was observed surrounding the implanted composite and the inflammatory response of the host to the implant was mild. Specific proteoglycans were accumulated in the composite even 1 week after implantation. At 2 weeks after implantation, the chondrocytes regenerated the cartilage tissue, although still immature, but at 4 weeks almost all of the chondrocytes transferred to the mature stage. Conversely, such mature cartilage tissue was not noticed up to 4 weeks after implantation if the collagen scaffold was not impregnated with bFGF. Moreover, the mature area was limited to only a small fraction of the implanted composite, unless bFGF was incorporated in it.