Rhamnogalacturonan-II cross-linking of plant pectins via boron bridges occurs during polysaccharide synthesis and/or secretion.
Rhamnogalacturonan-II cross-linking of plant pectins via boron bridges occurs during polysaccharide synthesis and/or secretion.
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DOI：
10.4161/psb.28169
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发表时间：
2014
影响因子：
2.9
通讯作者：
Fry SC
中科院分区：
文献类型：
--
作者：
Chormova D;Messenger DJ;Fry SC
鼠李糖半乳糖醛酸-II（RG-II）是植物细胞壁果胶的一个结构域，能够通过硼酸二酯桥与其他RG-II结构域交联。虽然已知它会影响细胞壁的机械性能，但这种交联的生物化学要求和生命周期仍不清楚。我们开发了一种PAGE方法，使单体和二聚体的RG-II的分离，并使用它来研究在体外和体内的交联动力学。在没有添加硼的培养基中生长的Rosa细胞不含RG-II二聚体，尽管这些二聚体在向培养基中添加硼后重新出现。然而，不能合成新多糖的其它蔷薇属培养物没有显示二聚体形成。我们的结论是，RG-II通常成为交联intraprotoplasmically或在分泌过程中，但不是分泌后。
Rhamnogalacturonan-II (RG-II), a domain of plant cell wall pectins, is able to cross-link with other RG-II domains through borate diester bridges. Although it is known to affect mechanical properties of the cell wall, the biochemical requirements and lifecycle of this cross-linking remain unclear. We developed a PAGE methodology to allow separation of monomeric and dimeric RG-II and used this to study the dynamics of cross-linking in vitro and in vivo. Rosa cells grown in medium with no added boron contained no RG-II dimers, although these re-appeared after addition of boron to the medium. However, other Rosa cultures which were unable to synthesize new polysaccharides did not show dimer formation. We conclude that RG-II normally becomes cross-linked intraprotoplasmically or during secretion, but not post-secretion.