THE UREASE ENZYMES OF CAMPYLOBACTER-PYLORI AND A RELATED BACTERIUM
THE UREASE ENZYMES OF CAMPYLOBACTER-PYLORI AND A RELATED BACTERIUM
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DOI：
10.1099/00222615-27-1-33
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发表时间：
1988-09-01
影响因子：
3
通讯作者：
LEE, A
中科院分区：
文献类型：
--
作者：
FERRERO, RL;HAZELL, SL;LEE, A
对幽门弯曲菌的尿素酶进行了研究，并与从啮齿动物回肠中分离的相关螺旋形细菌STL的尿素酶进行了比较。这两种细菌都具有构成尿素酶的活性，其活性可达普通变形杆菌的20-70倍。这一活性被保留在十二烷基硫酸钠-聚丙烯酰胺凝胶上。摩尔的一个主要催化亚基。经SDS-PAGE分析，6株幽门螺杆菌均定位于300000，而STL有两种活性形式的分子。WTS为14万和15万。免疫印迹分析表明，幽门螺杆菌相关性胃炎患者血清中存在抗尿素酶抗体。对幽门螺杆菌尿素酶的反应不是菌株特异性的，但在幽门螺杆菌和STL之间没有发现交叉反应。这些细菌的极高的尿素酶活性可能对宿主的定殖很重要。这两种生物都具有谷氨酸脱氢酶活性，这表明尿素酶的一个作用可能是同化可利用的尿素氮。另一个可能的功能是改善当地环境，以促进长期殖民。对酸的保护不太可能是主要作用，因为生物体的自然栖息地STL是小肠的非酸分泌组织。
The urease enzyme of Campylobacter pylori was studied and compared with that of a related spiral-shaped bacterium, Stl, isolated from the rodent ileum. Both bacteria possessed constitutive urease enzymes with activities up to 20-70 times that of Proteus vulgaris. This activity was retained on SDS-polyacrylamide gels. A major catalytic subunit of mol. wt 300 000 was located for all (six) strains of C. pylori subjected to SDS-PAGE whereas Stl had two active forms of mol. wts 140 000 and 150 000. Western-blot analysis indicated the presence of anti-urease antibodies in the sera of patients with C. pylori-associated gastritis. The response to C. pylori urease was not strain-specific but no cross-reactivity was detected between the C. pylori enzyme and that of Stl. The very high urease activity of these bacteria is likely to be important in colonisation of the host. Possession of glutamate dehydrogenase activity by both organisms suggest that one role of the urease may be to assimilate the available urea nitrogen. Modification of the local environment to facilitate long-term colonisation is another possible function. Protection from acid is unlikely to be a primary role as the natural habitat of the organism Stl is the non-acid-secreting tissue of the small intestine.