Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole
Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole
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DOI：
10.1016/j.biortech.2011.10.041
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发表时间：
2012-01-01
影响因子：
11.4
通讯作者：
Nishida, Tomoaki
中科院分区：
文献类型：
--
作者：
Suda, Tomoyo;Hata, Takayuki;Nishida, Tomoaki
四环素类抗生素广泛用于人类和兽医学;然而，这些抗生素在环境中的残留量令人担忧，因为它们可能有助于选择耐药细菌。在氧化还原介体1-羟基苯并三唑（HBT）存在下，用来自白色真菌变色栓菌（Trametesversicolor）的漆酶处理四环素（TC）、金霉素（CTC）、强力霉素（DC）和土霉素（OTC）。高效液相色谱显示DC和CTC在15 min后完全消除，而TC和CTC在1 h后完全消除。随着四环素抗生素浓度的降低，该系统也导致对大肠杆菌和枯草芽孢杆菌以及绿色假基氏菌的生长抑制完全丧失。这些结果表明，漆酶-HBT体系对四环素类抗生素具有良好的去除效果，并能有效地去除四环素类抗生素的生态毒性。(C)2011爱思唯尔有限公司保留所有权利。
Tetracycline antibiotics are widely used in human and veterinary medicine; however, residual amounts of these antibiotics in the environment are of concern since they could contribute to selection of resistant bacteria. In this study, tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) and oxytetracycline (OTC) were treated with laccase from the white rot fungus Trametes versicolor in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). High performance liquid chromatography demonstrated that DC and CTC were completely eliminated after 15 min, while TC and CTC were eliminated after 1 h. This system also resulted in a complete loss of inhibition of growth of Escherichia coli and Bacillus subtilis and the green alga Pseudokirchneriella subcapitata with decreasing tetracycline antibiotic concentration. These results suggest that the laccase-HBT system is effective in eliminating tetracycline antibiotics and removing their ecotoxicity. (C) 2011 Elsevier Ltd. All rights reserved.