Ubiquitin deposits are present in spinal motor neurons in all stages of the disease in the motor neuron degeneration (Mnd) mutant of the mouse.
Ubiquitin deposits are present in spinal motor neurons in all stages of the disease in the motor neuron degeneration (Mnd) mutant of the mouse.
复制标题
在小鼠运动神经元变性（Mnd）突变体疾病的所有阶段，泛素沉积物都存在于脊髓运动神经元中。
DOI：
10.1016/0304-3940(91)90256-s
复制
发表时间：
1991
影响因子：
2.5
通讯作者：
Mazurkiewicz,JE
中科院分区：
文献类型：
--
作者：
Mazurkiewicz,JE
使用泛素单克隆抗体对 Mnd（运动神经元变性）小鼠（运动神经元疾病的动物模型）的脊髓进行免疫细胞化学分析。对患有轻度、中度和重度疾病的小鼠、症状前小鼠和年龄匹配的对照小鼠的组织进行了分析。在症状前动物和疾病所有阶段的脊髓神经元中观察到泛素沉积。在所用抗体的浓度下，在对照小鼠中没有观察到免疫反应性沉积物。症状前脊髓运动神经元中泛素免疫阳性的存在表明泛素化可能在小鼠运动神经元疾病的发病机制中发挥主要作用，并且可能在一般运动神经元疾病的发病机制中发挥主要作用。
Monoclonal antibodies to ubiquitin were used in an immunocytochemical analysis of spinal cord from theMnd(motor neuron degeneration) mouse, an animal model for motor neuron disease. Tissue from mice with mild, moderate and severe disease, from presymptomatic mice, and agematched controls were analyzed. Ubiquitin deposits were observed in spinal neurons from presymptomatic animals and all stages of the disease. No immunoreactive deposits were observed in control mice at the concentration of the antibodies used. The presence of ubiquitin immunopositivity in presymptomatic spinal motor neurons suggests that ubiquitination might play a primary role in the pathogenesis of motor neuron disease in theMndmouse, and perhaps of motor neuron disease in general.