Biomimetic injectable hydrogel based on silk fibroin/hyaluronic acid embedded with methylprednisolone for cartilage regeneration.
Biomimetic injectable hydrogel based on silk fibroin/hyaluronic acid embedded with methylprednisolone for cartilage regeneration.
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DOI：
10.1016/j.colsurfb.2022.112859
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发表时间：
2022-09
期刊：
影响因子：
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通讯作者：
V. Phan;Mohanapriya Murugesan;P. P. T. Nguyen-P.;Cuong Hung Luu;Ngoc-Han Hoai Le;H. Nguyen;P. Manivasagan;Eue-Soon Jang;Yi Li;Thavasyappan Thambi
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文献类型：
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作者：
V. Phan;Mohanapriya Murugesan;P. P. T. Nguyen-P.;Cuong Hung Luu;Ngoc-Han Hoai Le;H. Nguyen;P. Manivasagan;Eue-Soon Jang;Yi Li;Thavasyappan Thambi
关节软骨损伤的特点是由于缺乏血管、淋巴管和神经，自我修复能力有限。因此，本研究旨在开发一种经典的可注射水凝胶平台，能够以类似于自然细胞外微环境的微创手术修复软骨缺损，并为细胞的黏附和增殖提供高孔度的网络。为了适应上述要求，本研究开发了一种由丝素蛋白(SF)和透明质酸(HA)组成的可注射支架系统。此外，将甲基强的松龙(MP)包裹在SF/HA支架上，以减轻炎症反应。将SF中的赖氨酸残基通过席夫碱的形成进行化学交联，制备出孔径为100.47±32.09µm的SF/HA水凝胶支架，水凝胶的高孔性进一步有利于软组织的再生。在鸡胚绒毛尿囊膜(CAM)的体外实验中，SF/HA水凝胶不改变血管的生成和血管的形成，因此适合于软骨再生。此外，在小鼠模型中验证了体内凝胶的形成，表明SF/HA水凝胶的原位凝胶形成。更重要的是，SF/HA水凝胶表现出可控的生物降解性。综上所述，SF/HA水凝胶为制备有效的组织再生支架提供了进一步的启示，为提高关节软骨损伤的治疗水平铺平了道路。
Articular cartilage injury is characterized by limited self-repair capacity due to the shortage of blood vessels, lymphatics, and nerves. Hence, this study aims to exploit a classic injectable hydrogel platform that can restore the cartilage defects with minimally invasive surgery, which is similar to the natural extracellular microenvironment, and highly porous network for cell adhesion and proliferation. In this study, an injectable scaffold system comprised of silk fibroin (SF) and hyaluronic acid (HA) was developed to adapt the above requirements. Besides, methylprednisolone (MP) was encapsulated by SF/HA scaffold for alleviating inflammation. The SF/HA hydrogel scaffold was prepared by chemical cross-linking between the lysine residues of SF via Schiff base formation, and pore diameter of the obtained hydrogels was 100.47 ± 32.09 µm. The highly porous nature of hydrogel could further benefit the soft tissue regeneration. Compared with HA-free hydrogels, SF/HA hydrogel showed more controlled release on MP.In ovoexperiment of chick embryo chorioallantoic membrane (CAM) demonstrated that SF/HA hydrogels not altered the angiogenesis and formation of blood vessels, thus making it suitable for cartilage regeneration. Furthermore, in vivo gel formation was validated in mice model, suggesting in situ gel formation of SF/HA hydrogels. More importantly, SF/HA hydrogels exhibited the controlled biodegradation. Overall, SF/HA hydrogels provide further insights to the preparation of effective scaffold for tissue regeneration and pave the way to improve the articular cartilage injury treatment.