RNAS FROM 2 INDEPENDENTLY ISOLATED DEFECTIVE INTERFERING PARTICLES OF SINDBIS VIRUS CONTAIN A CELLULAR TRANSFER-RNA SEQUENCE AT THEIR 5' ENDS
RNAS FROM 2 INDEPENDENTLY ISOLATED DEFECTIVE INTERFERING PARTICLES OF SINDBIS VIRUS CONTAIN A CELLULAR TRANSFER-RNA SEQUENCE AT THEIR 5' ENDS
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DOI：
10.1073/pnas.80.11.3279
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
SCHLESINGER, S
中科院分区：
文献类型：
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作者：
MONROE, SS;SCHLESINGER, S
缺陷干扰（DI）颗粒是特异性干扰同源标准病毒复制的缺失突变体。通过以下方法确定2个DI RNA群体的5“-末端核苷酸序列：从DI RNA群体之一克隆c[互补]DNA，并对代表性克隆进行测序，并在模板处使用两个DI RNA群体制备引物指导的cDNA转录物并对这些转录物进行测序。2个DI RNA群体的5“末端序列不是来源于标准辛德毕斯病毒RNA，而是与细胞tRNAAsp的序列几乎相同。
Defective interfering (DI) particles are deletion mutants that interfere specifically with the replication of homologous standard virus. The 5''-terminal nucleotide sequences of 2 DI RNA populations were determined by the following methods: cloning of the c[complementary]DNA from one of the DI RNA populations and sequencing a representative clone and using both DI RNA populations at templates for preparing primer-directed cDNA transcripts and sequencing these transcripts. The 5'' terminal sequences of the 2 DI RNA populations were not derived from standard Sindbis viral RNA but were almost identical to those of a cellular tRNAAsp.