Staphylococcus aureus and Bacillus subtilis W23 Make Polyribitol Wall Teichoic Acids Using Different Enzymatic Pathways
Staphylococcus aureus and Bacillus subtilis W23 Make Polyribitol Wall Teichoic Acids Using Different Enzymatic Pathways
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DOI：
10.1016/j.chembiol.2010.07.017
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发表时间：
2010-10-29
影响因子：
--
通讯作者：
Walker, Suzanne
中科院分区：
文献类型：
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作者：
Brown, Stephanie;Meredith, Timothy;Walker, Suzanne
壁磷壁酸（WTA）是在细菌细胞形状、细胞分裂、包膜完整性、生物膜形成致病性B subtilis W23和金黄色葡萄球菌都能产生多核糖醇磷酸（RboP）我们使用体外重组结合遗传学的方法表明，在B subtilis W23和金黄色葡萄球菌中，WTA生物合成的途径是不同的。金黄色葡萄球菌需要称为TarF的甘油磷酸引发酶以产生RboP-WTA，B subtilis W23含有TarF同系物，但该酶产生甘油磷酸聚合物并且不参与RboP-WTA合成。相反，B subtilis TarK代替TarF发挥功能以引发WTA中间体用于TarL的链延伸。
Wall teichoic acids (WTAs) are anionic polymers that play key roles in bacterial cell shape, cell division, envelope integrity, biofilm formation, and pathogenesis B subtilis W23 and S aureus both make polyribitol-phosphate (RboP) WTAs and contain similar sets of biosynthetic genes We use in vitro reconstitution combined with genetics to show that the pathways for WTA biosynthesis in B subtilis W23 and S aureus are different S aureus requires a glycerol-phosphate primase called TarF in order to make RboP-WTAs, B subtilis W23 contains a TarF homolog, but this enzyme makes glycerol-phosphate polymers and is not involved in RboP-WTA synthesis Instead, B subtilis TarK functions in place of TarF to prime the WTA intermediate for chain extension by TarL This work highlight:, the enzymatic diversity of the poorly characterized family of phosphotransferases involved in WTA biosynthesis in Gram-positive organisms