Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni.
Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni.
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成年叙利亚仓鼠感染空肠弯曲杆菌鞭毛变种。
DOI：
10.1128/iai.58.7.2214-2219.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
Nachamkin,I
中科院分区：
文献类型：
--
作者：
Aguero-Rosenfeld,ME;Yang,XH;Nachamkin,I
测试了在鞭毛表达方面不同的空肠弯曲杆菌IN 1的两种变体IN 1（弗拉+ Mot+，野生型）和IN 1-NM（弗拉- Mot-）在成年仓鼠中建立感染的能力。用2 × 10（9）至5 × 10（10）CFU对动物进行盲肠内攻毒，并监测感染迹象。攻毒动物均未出现疾病。然而，IN 1感染仓鼠的能力（43只中的35只）与IN 1-NM感染仓鼠的能力（42只中的1只）相比存在显著差异（P <0.01）。此外，用IN 1-NM激发的8只动物仅分泌弗拉+表型的弯曲杆菌，其显示与亲本弗拉+ Mot+型相同。通过酶联免疫吸附试验测定，两组动物均产生了针对IN 1外膜蛋白的血清免疫球蛋白G抗体;然而，仅分泌弗拉+生物体的动物产生了抗鞭毛蛋白抗体。从弗拉+到弗拉-的体外可逆性发生的频率为9.2 × 10（-6）/细胞/代;然而，从弗拉-到弗拉+的逆转不能在体外检测到。IN 1-NM的进一步表征表明，它产生的细胞质62 K鞭毛蛋白亚基蛋白，但这种蛋白质缺乏六个表位检测在IN 1中，也不同，在其二维凝胶电泳模式。这些研究结果支持了完整的鞭毛是肠道感染C.该模型可用于研究对空肠弯曲菌的免疫应答。空肠。
Two variants of Campylobacter jejuni IN1 differing in the expression of flagella, IN1 (Fla+ Mot+, wild type) and IN1-NM (Fla- Mot-), were tested for their ability to establish infection in adult hamsters. Animals were challenged intracecally with 2 X 10(9) to 5 X 10(10) CFU and monitored for evidence of infection. None of the challenged animals developed illness. There was a significant difference, however, in the ability of IN1 to infect hamsters (35 of 43) compared with that of IN1-NM (1 of 42) (P less than 0.01). Additionally, eight animals challenged with IN1-NM excreted only the campylobacters of Fla+ phenotype, which were shown to be identical with the parental Fla+ Mot+ type. Both groups of animals developed serum immunoglobulin G antibodies to outer membrane proteins of IN1 as measured by an enzyme-linked immunosorbent assay; however, only animals that excreted Fla+ organisms developed antiflagellin antibodies. In vitro reversibility from Fla+ to Fla- occurred with a frequency of 9.2 X 10(-6) per cell per generation; however, reversion from Fla- to Fla+ could not be detected in vitro. Further characterization of IN1-NM showed that it produced a cytoplasmic 62K flagellin subunit protein, but this protein lacked six epitopes detected in IN1 and also differed in its two-dimensional gel electrophoresis pattern. The results of these studies support the concept that an intact flagellum is necessary for intestinal infection with C. jejuni and that this model may be useful for studying the immune response to C. jejuni.