Analysis of gene expression in two human-derived cell lines exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field
Analysis of gene expression in two human-derived cell lines exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field
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DOI：
10.1002/pmic.200700215
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发表时间：
2007-11-01
期刊：
影响因子：
3.4
通讯作者：
McNamee, James P.
中科院分区：
文献类型：
--
作者：
Chauhan, Vinita;Qutob, Sami S.;McNamee, James P.
围绕移动的电话发射的射频场的生物效应存在相当大的争议。我们实验室先前的工作表明，暴露于1.9 GHz脉冲调制射频场对人胶质母细胞瘤衍生细胞系（U87 MG）中22 000个基因的表达没有影响，在6 h后暴露4 h射频场。作为本研究的后续，我们现在已经检查了RF场暴露对24小时RF暴露期后U87 MG细胞中迟发型基因的可能表达的影响。此外，将人单核细胞衍生的细胞系（Mono-Mac-6，MM 6）暴露于间歇性（5 min ON，10 min OFF）RF场6 h，然后在暴露后即刻和暴露后18 h评估基因表达。将两种细胞系暴露于1.9 GHz脉冲调制RF场6或24 h，比吸收率（SAR）为0.1-10.0 W/kg。在支持我们以前的结果，我们没有发现任何证据表明，非热射频场暴露可以改变基因表达在培养的U87 MG或MM 6细胞，相对于非辐照对照组。然而，这两种细胞系暴露于热休克条件下（43 ℃ 1小时）引起了一些充分表征的热休克蛋白表达的改变。
There is considerable controversy surrounding the biological effects of radiofrequency (RF) fields, as emitted by mobile phones. Previous work from our laboratory has shown no effect related to the exposure of 1.9 GHz pulse-modulated RF fields on the expression of 22 000 genes in a human glioblastoma-derived cell-line (U87MG) at 6 h following a 4 h RF field exposure period. As a follow-up to this study, we have now examined the effect of RF field exposure on the possible expression of late onset genes in U87MG cells after a 24 h RF exposure period. In addition, a human monocyte-derived cell-line (Mono-Mac-6, MM6) was exposed to intermittent (5 min ON, 10 min OFF) RF fields for 6 h and then gene expression was assessed iinmediately after exposure and at 18 h postexposure. Both cell lines were exposed to 1.9 GHz pulse-modulated RF fields for 6 or 24 h at specific absorption rates (SARs) of 0.1-10.0 W/kg. In support of our previous results, we found no evidence that nonthermal RF field exposure could alter gene expression in either cultured U87MG or MM6 cells, relative to nonirradiated control groups. However, exposure of both cell-lines to heat-shock conditions (43 degrees C for 1 h) caused an alteration in the expression of a number of well-characterized heat-shock proteins.