Dental Pulp Cells for Induced Pluripotent Stem Cell Banking
Dental Pulp Cells for Induced Pluripotent Stem Cell Banking
复制标题
DOI：
10.1177/0022034510366846
复制
发表时间：
2010-08-01
影响因子：
7.6
通讯作者：
Tezuka, K.
中科院分区：
文献类型：
--
作者：
Tamaoki, N.;Takahashi, K.;Tezuka, K.
定义的转录因子组可以将人类体细胞重编程为诱导多能干细胞（iPS）。然而，许多类型的人类细胞不容易进行微创手术。在这里，我们评估了牙髓细胞（DPC）作为iPS细胞的最佳来源，因为它们很容易从拔牙中获得，并且可以在简单的培养条件下扩增。从用常规的3或4重编程因子测试的所有6个DPC系中，从5个DPC系有效地建立了iPS细胞。此外，对107个DPC系的HLA类型的测定显示，2个系对所有3个HLA基因座都是纯合的，并表明如果从这些初始库建立iPS库，该库将覆盖约20%的日本人口，具有完美匹配。对这些数据的分析表明，DPC收集物作为用于再生医学的iPS细胞库的来源具有很大的潜力。
Defined sets of transcriptional factors can reprogram human somatic cells to induced pluripotent stem (iPS) cells. However, many types of human cells are not easily accessible to minimally invasive procedures. Here we evaluated dental pulp cells (DPCs) as an optimal source of iPS cells, since they are easily obtained from extracted teeth and can be expanded under simple culture conditions. From all 6 DPC lines tested with the conventional 3 or 4 reprogramming factors, iPS cells were effectively established from 5 DPC lines. Furthermore, determination of the HLA types of 107 DPC lines revealed 2 lines homozygous for all 3 HLA loci and showed that if an iPS bank is established from these initial pools, the bank will cover approximately 20% of the Japanese population with a perfect match. Analysis of these data demonstrates the promising potential of DPC collections as a source of iPS cell banks for use in regenerative medicine.