Characterization of anti-myocardial autoantibodies in Japanese patients with dilated cardiomyopathy.
Characterization of anti-myocardial autoantibodies in Japanese patients with dilated cardiomyopathy.
复制标题
日本扩张型心肌病患者抗心肌自身抗体的特征。
DOI：
10.1253/jcj.65.867
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
S. Ogawa
中科院分区：
文献类型：
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作者：
A. Baba;T. Yoshikawa;M. Chino;A. Murayama;K. Mitani;S. Nakagawa;I. Fujii;M. Shimada;M. Akaishi;S. Iwanaga;Y. Asakura;K. Fukuda;H. Mitamura;S. Ogawa
以往很少有报道全面筛查特发性扩张型心肌病（IDC）患者的所有抗心肌自身抗体（AMCA）与其他临床特征的关系，因此本研究采用免疫组化（FITC）和免疫印迹（IB）筛查IDC患者，以表征AMCA的临床意义。收集100例IDC患者和100例年龄匹配的健康对照者（CTL）的血清。对于FITC，将人心肌的未固定冷冻切片用于标准间接免疫荧光;对于IB，将相同心肌的总心脏匀浆以2组稀释度（1：200和1：10，000）印迹至血清。各方法AMCA检测阳性率如下（IDC vs CTL）：FITC为39% vs 6%，IB（1：200）为38% vs 4%，IB（1：10，000）为10% vs 0%。59例IDC和8例CTL同时检测AMCA，18例IDC和2例CTL同时检测AMCA。通过对非持续性室性心动过速以及左心室舒张末期直径和血浆去甲肾上腺素浓度进行多元logistic回归分析，1：200时的IB阳性是一个独立的预测因子。
Few previous reports have comprehensively screened all the anti-myocardial autoantibodies (AMCA) in relation to other clinical profiles in patients with idiopathic dilated cardiomyopathy (IDC), so the present study used both immunohistochemistry (FITC) and immunoblotting (IB) for screening patients with IDC in order to characterize the clinical significance of AMCA. Sera were collected from 100 patients with IDC and age-matched 100 healthy control subjects (CTL). For FITC, an unfixed frozen section of human myocardium was used for the standard indirect immunofluorescence; for IB, total cardiac homogenates of the same myocardium were blotted to serum at 2 sets of dilution (1:200 and 1:10,000). The positive rates of AMCA detection for each method were as follows (IDC vs CTL); 39% vs 6% for FITC, 38% vs 4% for IB (1:200), and 10% vs 0% for IB (1:10,000). Fifty-nine patients with IDC and 8 CTL were positive for AMCA by either method, and 18 patients with IDC and 2 CTL were positive for AMCA by both methods. IB-positivity at 1:200 was an independent predictor by multiple logistic regression analysis of non-sustained ventricular tachycardias as well as left ventricular end-diastolic diameter and plasma norepinephrine concentration.