Arsenic speciation in marine product samples: Comparison of extraction-HPLC method and digestion-cryogenic trap method
Arsenic speciation in marine product samples: Comparison of extraction-HPLC method and digestion-cryogenic trap method
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DOI：
10.1016/j.talanta.2009.03.067
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发表时间：
2009-07-15
期刊：
影响因子：
6.1
通讯作者：
Ohki, Akira
中科院分区：
文献类型：
--
作者：
Geng, Wenhua;Komine, Rieko;Ohki, Akira
针对海产品样品中砷的形态分析，比较了两种预处理-分析组合。一种是结合溶剂萃取和高效液相色谱法（HPLC），然后进行高灵敏度的砷检测，而另一种是结合碱消化和低温阱（CT）的方法，然后进行高灵敏度的砷检测。用于六种海洋动物样品的标准物质。从萃取-HPLC方法获得的砷甜菜碱（AsB）的浓度与通过消化-CT方法测量的三甲基化砷物质的浓度非常一致。采用萃取-HPLC法对4种海藻样品中的3种砷糖（糖-1、糖-2和糖-3）进行了测定。这些海藻样品也经受消化-CT方法，并且通过该方法测量的二甲基化砷物种的量近似等于从提取-HPLC方法获得的二甲基胂酸（DMAA）和三种砷糖（糖-1+糖-2+糖-3）的量的总和。(C)2009爱思唯尔有限公司版权所有。
For the arsenic speciation in marine product samples, two types of pretreatment-analysis combination were compared. One is the combination of solvent extraction and high performance liquid chromatography (HPLC) followed by a highly sensitive arsenic detection, while the other is the combination of alkaline digestion and cryogenic trap (CT) method followed by a highly sensitive arsenic detection. For six certified reference materials (CRMs) of marine animal samples. the concentrations of arsenobetaine (AsB) obtained from the extraction-HPLC method were very consistent with those of trimethylated arsenic species measured by the digestion-CT method. For four seaweed samples, the determination of three arsenosugars (Sugar-1, Sugar-2, and Sugar-3) was favorably carried out by the extraction-HPLC method. Those seaweed samples were also subjected to the digestion-CT method, and the amounts of dimethylated arsenic species measured by the method were approximately equal to the sum of the amounts of dimethylarsinic acid (DMAA) and three arsenosugars (Sugar-1 + Sugar-2 + Sugar-3) obtained from the extraction-HPLC method. (C) 2009 Elsevier B.V. All rights reserved.