The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights.
The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights.
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DOI：
10.1002/oby.23081
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Armstrong SC
中科院分区：
文献类型：
--
作者：
McCann JR;Bihlmeyer NA;Roche K;Catherine C;Jawahar J;Kwee LC;Younge NE;Silverman J;Ilkayeva O;Sarria C;Zizzi A;Wootton J;Poppe L;Anderson P;Arlotto M;Wei Z;Granek JA;Valdivia RH;David LA;Dressman HK;Newgard CB;Shah SH;Seed PC;Rawls JF;Armstrong SC
建立肥胖青少年的临床、代谢组学和微生物组样本的生物储存库，因为他们正在接受生活方式的改变。我们招募了223名10-18岁的青少年，体重指数≥第95百分位数，沿着71名健康体重的参与者。我们在6个月内重复收集临床数据、空腹血清和粪便样本。在这里，我们介绍了我们的研究设计，数据收集方法和中期分析，包括有针对性的血清代谢物测量和粪便16 S rRNA基因扩增子测序青少年肥胖（n=27）和健康体重控制（n=27）。与健康体重对照组相比，肥胖青少年的血清丙氨酸氨基转移酶、C反应蛋白和糖化血红蛋白较高，高密度脂蛋白胆固醇较低。代谢组学显示支链氨基酸相关代谢物的差异。我们还观察到与健康体重组相比，肥胖青少年中特定微生物类群的丰度差异和物种多样性降低。杜克儿科代谢和微生物组研究生物储存库可作为共享资源使用。早期研究结果表明，青少年特有的肥胖代谢特征的证据，沿着先前报道的描述肥胖代谢和微生物组标志物的研究结果的确认。观察性试验：NCT 03139877
To establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. We enrolled 223 adolescents aged 10–18 years with Body Mass Index ≥ 95th percentile, along with 71 healthy weight participants. We collected clinical data, fasting serum, and fecal samples at repeated intervals over 6 months. Here we present our study design, data collection methods, and interim analysis, including targeted serum metabolite measurements and fecal 16S rRNA gene amplicon sequencing among adolescents with obesity (n=27) and healthy weight controls (n=27). Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched chain amino acid-related metabolites. We also observed differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. The Duke Pediatric Metabolism and Microbiome Study biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings describing metabolic and microbiome markers of obesity. Biorepository: NCT02959034 Observational Trial: NCT03139877
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影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
影响因子：
4.5
作者：
Ferrara CT;Wang P;Neto EC;Stevens RD;Bain JR;Wenner BR;Ilkayeva OR;Keller MP;Blasiole DA;Kendziorski C;Yandell BS;Newgard CB;Attie AD
通讯作者：
Attie AD
影响因子：
8
作者：
Barlow, Sarah E.
通讯作者：
Barlow, Sarah E.
影响因子：
8
作者：
通讯作者：
--
影响因子：
1.6
作者：
Dolinsky, Diana H.;Armstrong, Sarah C.;Kemper, Alex R.
通讯作者：
Kemper, Alex R.