Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
复制标题
拟南芥中 AhGLK1b（GOLDEN2 样转录因子）的异位表达赋予对真菌和细菌病原体的双重抗性
DOI：
10.3390/genes11030343
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发表时间：
2020-03-01
期刊：
影响因子：
3.5
通讯作者：
Zhuang, Weijian
中科院分区：
文献类型：
--
作者：
Ali, Niaz;Chen, Hua;Zhuang, Weijian
GOLDEN2样蛋白(GOLDEN2-like，GLK)是髓母细胞增生症(MYB)家族的一员，在调节胞质发育和逆境耐受中发挥重要作用。在本研究中，从栽培花生中发现了一个基因AhGLK1b，该基因在低钙胁迫下表达下调，其完整的开放阅读框(ORF)为1212个碱基对。AhGLK1b与花生和杜兰的同源性分别为99.26%和96.28%。在花生中，AhGLK1b定位于细胞核，在叶片中的表达最高，其次是胚。此外，AhGLK1b的表达受到青枯雷尔氏菌侵染的显著诱导。AhGLK1b在拟南芥中的异位表达对重要的植物病原真菌核盘菌表现出较强的抗性。对细菌病原菌PST DC3000的侵染也表现出较高的抗性。表达AhGLK1b的拟南芥诱导了包括Pr10和Phox/Bem 1(PBI)在内的防御相关基因，它们参与了多种抗病反应。综上所述，这些结果表明AhGLK1b在提供对真菌和细菌病原体的双重抗性以及对非生物胁迫的耐受性方面可能是有用的。
GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a complete open reading frame (ORF) of 1212 bp. The AhGLK1b has 99.26% and 96.28% sequence similarities with its orthologs in Arachis ipaensis and A. duranensis, respectively. In the peanut, the AhGLK1b was localized in the nucleus and demonstrated the highest expression in the leaf, followed by the embryo. Furthermore, the expression of AhGLK1b was induced significantly in response to a bacterial pathogen, Ralstonia solanacearum infection. Ectopic expression of AhGLK1b in Arabidopsis showed stronger resistance against important phytopathogenic fungi S. sclerotiorum. It also exhibited high resistance to infection of the bacterial pathogen Pst DC3000. AhGLK1b-expressing Arabidopsis induced defense-related genes including PR10 and Phox/Bem 1 (PBI), which are involved in multiple disease resistance. Taken together, the results suggest that AhGLK1b might be useful in providing dual resistance to fungal and bacterial pathogens as well as tolerance to abiotic stresses.