Investigation of a Combined Microdroplet Generator and Pneumatic Nebulization System for Quantitative Determination of Metal-Containing Nanoparticles Using ICPMS
Investigation of a Combined Microdroplet Generator and Pneumatic Nebulization System for Quantitative Determination of Metal-Containing Nanoparticles Using ICPMS
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DOI：
10.1021/acs.analchem.5b01604
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发表时间：
2015-09-01
影响因子：
7.4
通讯作者：
Guenther, Detlef
中科院分区：
文献类型：
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作者：
Ramkorun-Schmidt, Benita;Pergantis, Spiros A.;Guenther, Detlef
在这项工作中，提出了一种常规适用的方法来表征金属纳米颗粒。将微滴发生器（MDG）产生的单个液滴合并到气动雾化器（PN）产生的气溶胶中，并引入ICPMS。MDG提供了单个和离散液滴的高传输效率，因此用于建立NP的质量定量的校准函数，所述NP是在如别处所述的单颗粒程序之后通过PN引入的。这种组合配置的主要优点包括大样品体积的快速处理，不同样品基质的快速交换，以及使用可追踪的元素标准品校准NP信号，从而避免了使用NP参比物质或其他不总是彻底表征的市售NP的需要。根据100%校准品通过MDG到达血浆的事实计算进样的转运效率，而对于PN，使用含有已知数量浓度的NP混悬液。或者，NP材料的批量分析允许在没有来自参考NP的任何额外信息的情况下确定转运效率。使用该方法，我们可以分别在60.4 +/- 1.0 nm和80.0 +/- 1.4 nm处测定标准银NP的尺寸，这与供应商给出的尺寸范围（60.8 +/- 6.6 nm和79.8 +/- 5.4 nm）一致。此外，我们还能够测定样品的NP数浓度（Ag/Au），与预期值的偏差为3.2%。
In this work, a routinely applicable approach is presented to characterize metal NPs. Individual droplets generated from a microdroplet generator (MDG) were merged into an aerosol generated by a pneumatic nebulizer (PN) and introduced into an ICPMS. The MDG offers high transport efficiency of individual and discrete droplets and was therefore used to establish a calibration function for mass quantification of NPs which were introduced through the PN following the single particle procedure as described elsewhere. The major advantages of such a combined configuration include fast processing of large sample volumes, fast exchanges of different sample matrixes, and the calibration of the NP signal using traceable elemental standards, thus avoiding the need to use NP reference materials or other, not always thoroughly characterized, commercially available NPs. The transport efficiency of the sample introduction is calculated based on the fact that 100% of the calibrant reaches the plasma through the MDG, whereas for the PN a NP suspension containing a known number concentration is used. Alternatively, bulk analysis of the NP material allows transport efficiency determination without any additional information from reference NPs. With this method, we could determine the size of standard silver NPs at 60.4 +/- 1.0 nm and 80.0 +/- 1.4 nm, respectively, which agrees with the size ranges given by the supplier (60.8 +/- 6.6 nm and 79.8 +/- 5.4 nm). Furthermore, we were also able to determine the NPs number concentration of the sample (Ag/Au) with a deviation of 3.2% the expected value.