Human Umbilical Cord-Derived Schwann-Like Cell Transplantation Combined with Neurotrophin-3 Administration in Dyskinesia of Rats with Spinal Cord Injury
Human Umbilical Cord-Derived Schwann-Like Cell Transplantation Combined with Neurotrophin-3 Administration in Dyskinesia of Rats with Spinal Cord Injury
复制标题
人脐带源性雪旺样细胞移植联合神经营养素3治疗脊髓损伤大鼠运动障碍
DOI：
10.1007/s11064-011-0402-9
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发表时间：
2011-05-01
影响因子：
4.4
通讯作者：
Duan Chuan-Zhi
中科院分区：
文献类型：
--
作者：
Guo Yan-Wu;Ke Yi-Quan;Duan Chuan-Zhi
间充质干细胞能够分化为雪旺细胞样细胞。在本研究中，我们在体外将人脐带间充质干细胞(HUMSCs)诱导分化为神经干细胞样细胞构成的神经球，并进一步分化为与雪旺样细胞在形态、表型和功能上具有很强相似性的细胞。HUMSC来源的雪旺样细胞移植到大鼠脊髓损伤(SCI)损伤区后，在改善运动功能方面显示出部分治疗效果。有报道称神经营养因子-3(NT-3)可以改善移植细胞的局部微环境，因此，我们进一步测试了雪旺样细胞移植联合NT-3在细胞移植部位的效果。结果表明，NT-3对移植细胞在宿主损伤部位的存活有明显的促进作用。在行为学测试中，雪旺细胞移植联合NT-3治疗的大鼠较单纯细胞移植的动物模型有明显改善。这些结果提示，雪旺细胞移植联合NT-3的应用可能是脊髓损伤干细胞治疗的一种新策略。
Mesenchymal stem cells are capable of differentiating into Schwann-like cells. In this study, we induced human umbilical-cord mesenchymal stem cells (HUMSCs) in vitro into neurospheres constituted by neural stem-like cells, and further into cells bearing strong morphological, phenotypic and functional resemblances with Schwann-like cells. These HUMSC-derived Schwann-like cells, after grafting into the injured area of the rats' spinal cord injury (SCI), showed a partial therapeutic effect in terms of improving the motor function. Neurotrophin-3 (NT-3) was reported to improve the local microenvironment of the grafted cells, and we, therefore, further tested the effect of Schwann-like cell grafting combined with NT-3 administration at the site of cell transplantation. The results showed that NT-3 administration significantly promoted the survival of the grafted cells in the host-injured area. Significant improvement in rats treated by Schwann-like cell grafting combined with NT-3 administration was demonstrated in the behavioral test as compared with that in animal models received the cell grafting only. These results suggest that transplantation of the Schwann-like cells combined with NT-3 administration may represent a new strategy of stem cell therapy for spinal cord injury.