Profiling of benzimidazoles and related metabolites in pig serum based on SiO2@NiO solid-phase extraction combined precursor ion scan with high resolution orbitrap mass spectrometry
Profiling of benzimidazoles and related metabolites in pig serum based on SiO2@NiO solid-phase extraction combined precursor ion scan with high resolution orbitrap mass spectrometry
复制标题
基于SiO2@NiO固相萃取结合前体离子扫描与高分辨率轨道阱质谱分析猪血清中苯并咪唑及相关代谢物
DOI：
10.1016/j.foodchem.2019.01.071
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Feng Yu-Qi
中科院分区：
文献类型：
--
作者：
Zheng Feng;Xiao Hua-Ming;Zhu Quan-Fei;Yu Qiong-Wei;Feng Yu-Qi
苯并咪唑类化合物（BZDs）是一种广泛应用于食用动物的兽药，其代谢产物在某些食品中残留。然而，BZD的某些代谢产物是未知的，因此发现新的代谢产物以扩大检测靶点是重要的。基于这些原因，我们设计了一种灵敏的、选择性的方法来鉴别猪口服阿苯达唑、芬苯达唑和噻苯达唑后血清中的BZD代谢物。将氧化镍纳米颗粒-二氧化硅（SiO2@NiO）复合物用于BZD化合物的富集和纯化。采用高效液相色谱-前体离子扫描-质谱（LC-PIS-MS）和高分辨率MS/MS分析（HR-MS/MS）对BZD代谢谱进行表征和代谢物检测。最终鉴定出18种BZD代谢产物，其中11种代谢产物为首次discovered.in猪血清中分离得到。此外，还提出了更全面的BZD代谢途径。
Benzimidazoles (BZDs) are widely used veterinary drugs in the domestic food-producing animals, resulting in the.metabolism of BZDs and the harmful metabolites residues in some foods. However, some BZD metabolites are.unknown and so it is important to discover new metabolites to expand detection targets. For these reasons, a.sensitive and selective strategy was designed to identify BZD metabolites in the serum of pigs after oral administration.of albendazole, fenbendazole and thiabendazole, respectively. Nickel oxide nanoparticle-deposited.silica (SiO2@NiO) composite was used for the enrichment and purification of BZD compounds. High-performance.liquid chromatography coupled with precursor ion scan-mass spectrometry (LC-PIS-MS) and high resolution.MS/MS analysis (HR-MS/MS) was employed for the BZDs metabolic profiles characterization and.metabolites detection. Finally, 18 BZD metabolites were identified, among which 11 metabolites were discovered.in pig serum for the first time. Besides, more comprehensive BZD metabolic pathways were presented.