Phospholipase Cγ2 is essential in the functions of B cell and several Fc receptors
Phospholipase Cγ2 is essential in the functions of B cell and several Fc receptors
复制标题
DOI：
10.1016/s1074-7613(00)00005-4
复制
发表时间：
2000-07-01
期刊：
影响因子：
32.4
通讯作者：
Ihle, JN
中科院分区：
文献类型：
--
作者：
Wang, DM;Feng, J;Ihle, JN
许多受体激活磷脂酶C γ 1或-γ 2。为了评估PLC γ 2的作用，我们获得了酶缺陷小鼠。小鼠存活，但成熟B细胞减少，前B细胞分化受阻，B1 B细胞缺乏。不存在IgM受体诱导的Ca 2+通量和向B细胞有丝分裂原的增殖。IgM、IgG 2a和IgG 3水平降低，并且不存在T细胞非依赖性抗体产生。与Btk或Blnk缺陷小鼠的相似性表明PLC γ 2在Btk/Blnk信号传导中处于下游。FcR γ信号传导也有缺陷，导致胶原蛋白诱导的血小板聚集、肥大细胞Fc γ R功能以及NK细胞Fc γ RIII和2B 4功能丧失。这些结果定义了免疫球蛋白超家族受体广泛利用的信号转导途径。
Many receptors activate phospholipase C gamma 1 or -gamma 2. To assess the role of PLC gamma 2, we derived enzyme-deficient mice. The mice are viable but have decreased mature B cells, a block in pro-B cell differentiation, and B1 B cell deficiency. IgM receptor-induced Ca2+ flux and proliferation to B cell mitogens are absent. IgM, IgG2a, and IgG3 levels are reduced, and T cell-independent antibody production is absent. The similarity to Btk- or Blnk-deficient mice demonstrates that PLC gamma 2 is downstream in Btk/Blnk signaling. FcR gamma signaling is also defective, resulting in a loss of collagen-induced platelet aggregation, mast cell Fc epsilon R function, and NK cell Fc gamma RIII and 2B4 function. The results define a signal transduction pathway broadly utilized by immunoglobulin superfamily receptors.