Acceleration of Purine Degradation by Periodontal Diseases
Acceleration of Purine Degradation by Periodontal Diseases
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DOI：
10.1177/0022034509341967
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发表时间：
2009-09-01
影响因子：
7.6
通讯作者：
Guo, L.
中科院分区：
文献类型：
--
作者：
Barnes, V. M.;Teles, R.;Guo, L.
牙周病，如牙龈炎和牙周炎，其特征在于牙龈缝隙周围的细菌菌斑积聚以及随后的宿主组织的炎症和破坏。为了检验宿主-细菌相互作用导致细胞代谢改变的假设，我们通过液相色谱和气相色谱质谱法对从健康、牙龈炎和牙周炎部位收集的人类龈沟液（GCF）进行了无偏倚的代谢组学分析。嘌呤降解途径是活性氧（ROS）产生的主要生化来源，在疾病部位显著加速。这表明牙周病引起的氧化应激和炎症是通过这一途径介导的。抗氧化剂的消耗、宿主细胞组分的降解和细菌产物在GCF中的积累进一步突出了复杂的宿主-细菌相互作用。这些发现为牙周病进展提供了新的机制见解和一组全面的生物标志物。
Periodontal diseases, such as gingivitis and periodontitis, are characterized by bacterial plaque accumulation around the gingival crevice and the subsequent inflammation and destruction of host tissues. To test the hypothesis that cellular metabolism is altered as a result of host-bacteria interaction, we performed an unbiased metabolomic profiling of gingival crevicular fluid (GCF) collected from healthy, gingivitis, and periodontitis sites in humans, by liquid and gas chromatography mass spectrometry. The purine degradation pathway, a major biochemical source for reactive oxygen species (ROS) production, was significantly accelerated at the disease sites. This suggests that periodontal-disease-induced oxidative stress and inflammation are mediated through this pathway. The complex host-bacterial interaction was further highlighted by depletion of anti-oxidants, degradation of host cellular components, and accumulation of bacterial products in GCF. These findings provide new mechanistic insights and a panel of comprehensive biomarkers for periodontal disease progression.