Bordetella pertussis whole cell immunization protects against Pseudomonas aeruginosa infections.
Bordetella pertussis whole cell immunization protects against Pseudomonas aeruginosa infections.
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百日咳全细胞免疫可预防铜绿假单胞菌感染。
DOI：
10.1038/s41541-022-00562-1
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发表时间：
2022-11-10
期刊：
影响因子：
9.2
通讯作者：
Barbier, Mariette
中科院分区：
文献类型：
--
作者：
Blackwood, Catherine B.;Mateu-Borras, Margalida;Sen-Kilic, Emel;Pyles, Gage M.;Miller, Sarah Jo;Weaver, Kelly L.;Witt, William T.;Huckaby, Annalisa B.;Kang, Jason;Chandler, Courtney E.;Ernst, Robert K.;Damron, F. Heath;Barbier, Mariette
全细胞疫苗是抗原、免疫原和有时佐剂的复杂混合物，其可以触发有效的和保护性的免疫应答。在某些情况下，例如全细胞百日咳博德特氏菌疫苗接种，对疫苗接种的免疫应答超出了疫苗预期针对的病原体，并有助于防止其他临床上重要的病原体。在这项研究中，我们描述了如何B。百日咳全细胞疫苗接种保护小鼠免受铜绿假单胞菌引起的急性肺炎。用ELISA和western blot方法鉴定了B。百日咳全细胞疫苗接种诱导产生结合铜绿假单胞菌的实验室适应性和临床菌株的抗体，而不管使用的免疫途径或佐剂。使用免疫沉淀、质谱和随后的免疫印迹鉴定交叉反应性抗原。我们确定B. B中存在百日咳GroEL和OmpA。百日咳全细胞疫苗分别导致产生针对铜绿假单胞菌GroEL和OprF的抗体。最后，我们证明了重组B。百日咳OmpA足以诱导针对铜绿假单胞菌急性鼠肺炎的保护。这项研究强调了使用B的潜力。百日咳OmpA作为预防铜绿假单胞菌感染的疫苗抗原，以及广泛保护性抗原用于疫苗开发的潜力。
Whole cell vaccines are complex mixtures of antigens, immunogens, and sometimes adjuvants that can trigger potent and protective immune responses. In some instances, such as whole cell Bordetella pertussis vaccination, the immune response to vaccination extends beyond the pathogen the vaccine was intended for and contributes to protection against other clinically significant pathogens. In this study, we describe how B. pertussis whole cell vaccination protects mice against acute pneumonia caused by Pseudomonas aeruginosa. Using ELISA and western blot, we identified that B. pertussis whole cell vaccination induces production of antibodies that bind to lab-adapted and clinical strains of P. aeruginosa, regardless of immunization route or adjuvant used. The cross-reactive antigens were identified using immunoprecipitation, mass spectrometry, and subsequent immunoblotting. We determined that B. pertussis GroEL and OmpA present in the B. pertussis whole cell vaccine led to production of antibodies against P. aeruginosa GroEL and OprF, respectively. Finally, we showed that recombinant B. pertussis OmpA was sufficient to induce protection against P. aeruginosa acute murine pneumonia. This study highlights the potential for use of B. pertussis OmpA as a vaccine antigen for prevention of P. aeruginosa infection, and the potential of broadly protective antigens for vaccine development.
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影响因子：
3.1
作者：
Fito-Boncompte, Laurene;Chapalain, Annelise;Chevalier, Sylvie
通讯作者：
Chevalier, Sylvie
影响因子：
11.1
作者：
Aaby, Peter;Andersen, Andreas;Zaman, K.
通讯作者：
Zaman, K.
影响因子：
11.8
作者：
Boucher, Helen W.;Talbot, George H.;Bartlett, John
通讯作者：
Bartlett, John
影响因子：
3.6
作者：
Aaby, Peter;Ravn, Henrik;Benn, Christine S.
通讯作者：
Benn, Christine S.
影响因子：
9.2
作者：
Boehm, Dylan T.;Wolf, M. Allison;Damron, F. Heath
通讯作者：
Damron, F. Heath