Kinetics of RANKL, RANK and OPG expressions in experimentally induced rat periapical lesions
Kinetics of RANKL, RANK and OPG expressions in experimentally induced rat periapical lesions
复制标题
DOI：
10.1016/j.tripleo.2006.11.036
复制
发表时间：
2007-05-01
期刊：
影响因子：
--
通讯作者：
Suda, Hideaki
中科院分区：
文献类型：
--
作者：
Kawashima, Nobuyuki;Suzuki, Noriyuki;Suda, Hideaki
Objective.本研究的目的是阐明实验诱导的大鼠根尖周病变中NF κ B B配体（RANKL）、RANK、骨保护素（OPG）和细胞因子表达的动力学。采用实时荧光定量PCR检测大鼠根尖周病变组织中RANKL、RANK、OPG和细胞因子的mRNA表达。通过暴露下第一磨牙的未封闭牙髓诱导雄性Wistar大鼠（n = 48，5周龄）的病变。RANKL的表达在病变扩展开始时上调，RANKL与RANKL的竞争者OPG的表达比率在2周和3周达到峰值。炎症细胞因子（如TNF-α、IL-1 α和IL-1 β）的表达也在此阶段增加，表明RANKL和促炎细胞因子信号传导对病变扩展的协同作用。RANKL（+）细胞以成纤维细胞为主，少数为T细胞。RANKL和促炎细胞因子的表达与根尖周病变的扩大相关。
Objective. The object of this study was to elucidate the kinetics of receptor activator of NF kappa B ligand (RANKL), RANK, osteoprotegerin (OPG), and cytokine expressions in experimentally induced rat periapical lesions.Study design. The mRNA expressions of RANKL, RANK, OPG, and cytokines in experimentally induced rat periapical lesions were evaluated by real-time PCR. The lesions were induced in male Wistar rats (n = 48, 5 weeks of age) by unsealed pulp exposure of the lower first molars.Results. Expression of RANKL was up-regulated at the beginning of lesion expansion, and expression ratio of RANKL against OPG, a competitor of RANKL, peaked at 2 and 3 weeks. Expression of inflammatory cytokines, such as TNF-alpha, IL-1 alpha, and IL-1 beta also increased at this stage, suggesting contribution of synergic effects of RANKL and proinflammatory cytokine signaling to lesion expansion. Most of RANKL(+) cells were fibroblastic, but few of them were T cells.Conclusion. Expression of RANKL and proinflammatory cytokines was correlated with periapical lesion expansion.