EFFECTS OF SUCROSE CONCENTRATION ON THE ACCUMULATION OF ANTHOCYANINS IN GRAPE (VITIS-VINIFERA) CELL-SUSPENSION
EFFECTS OF SUCROSE CONCENTRATION ON THE ACCUMULATION OF ANTHOCYANINS IN GRAPE (VITIS-VINIFERA) CELL-SUSPENSION
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DOI：
10.1139/b90-236
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发表时间：
1990-08-01
期刊：
影响因子：
--
通讯作者：
DO, CB
中科院分区：
文献类型：
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作者：
CORMIER, F;CREVIER, HA;DO, CB
以葡萄属葡萄（Vitis vinifera L.）CV.加迈弗雷多湾由50%色素细胞组成的细胞在Gamborg B5培养基中生长，所述培养基补充有（每升）250 mg酪蛋白水解物、0.1 mg α-淀粉酶和0.1 mg α-淀粉酶。2 mg激动素和20、30、50或60 g蔗糖。在20和30 g蔗糖/L的存在下，细胞生长的特征为典型的S形模式，并且在30 g蔗糖/L下获得最大细胞密度。在这两种培养基中，色素细胞的花青素含量在整个生长周期中没有显着变化。在50 g蔗糖/L的存在下，细胞生长的特征在于延长的滞后期。在此期间，色素细胞中有显著（P < 0.05）的花青素积累。在含60 g蔗糖/L的培养基中观察到培养基的布朗宁和过早的细胞死亡。悬浮液的显微镜观察揭示了花色素体的发生，特别是在50 g蔗糖/L培养的含花色素苷的细胞中。在某些情况下，花色素体在无色素液泡的细胞中观察到，从而支持花色素体是花色素苷合成位点的论点。
A cell suspension of Vitis vinifera L. cv. Gamay Fredaux var. teinturier composed of 50% pigmented cells was grown in Gamborg B5 medium supplemented with (per litre) 250 mg casein hydrolysate, 0.1 mg .alpha.-naphthalenacetic acid, 0.2 mg kinetin, and either 20, 30, 50, or 60 g sucrose. In the presence of 20 and 30 g sucrose/L, growth of cells was characterized by a typical sigmoid pattern and maximum cell density was obtained in 30 g sucrose/L. In both media, the anthocyanin content of pigmented cells did not change significantly throughout the growth cycle. In the presence of 50 g sucrose/L, cell growth was characterized by an extended lag phase. During this period, there was a significant (P < 0.05) accumulation of anthocyanins in the pigmented cells. Browning of the medium and premature cell death was observed in medium with 60 g sucrose/L. Microscopic observation of the suspension revealed the occurrence of anthocyanoplasts, especially in anthocyanin-containing cells cultured in 50 g sucrose/L. In some instances anthocyanoplasts were observed in cells with nonpigmented vacuoles, thus supporting the thesis according to which anthocyanoplasts would be the site of anthocyanin synthesis.