Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective
Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective
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DOI：
10.1364/ol.29.002521
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发表时间：
2004-11-01
期刊：
影响因子：
3.6
通讯作者：
Helmchen, F
中科院分区：
文献类型：
--
作者：
Göbel, W;Kerr, JND;Helmchen, F
我们提出了一个微型的，灵活的双光子显微镜组成的融合相干光纤束与30,000核心和梯度折射率透镜物镜。标准双光子激光扫描显微镜的激光焦点在光纤束的入口表面上扫描，导致顺序耦合到各个芯中。通过纤维束检测到荧光。微米大小的荧光珠和花粉粒很容易解决。此外，荧光标记的血管通过在体大鼠脑中的纤维束成像。(C)2004年美国光学学会。
We present a miniature, flexible two-photon microscope consisting of a fused coherent optical fiber bundle with 30,000 cores and a gradient-index lens objective. The laser focus of a standard two-photon laser-scanning microscope was scanned over the entrance surface of the fiber bundle, resulting in sequential coupling into individual cores. Fluorescent light was detected through the fiber bundle. Micrometer-sized fluorescent beads and pollen grains were readily resolved. In addition, fluorescently labeled blood vessels were imaged through the fiber bundle in rat brain in vivo. (C) 2004 Optical Society of America.