Role of peroxisome proliferator-activated receptor-alpha in fasting-mediated oxidative stress.
Role of peroxisome proliferator-activated receptor-alpha in fasting-mediated oxidative stress.
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DOI：
10.1016/j.freeradbiomed.2009.06.017
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发表时间：
2009-09-15
影响因子：
7.4
通讯作者：
Song, Byoung-Joon
中科院分区：
文献类型：
--
作者：
Abdelmegeed, Mohamed A.;Moon, Kwan-Hoon;Hardwick, James P.;Gonzalez, Frank J.;Song, Byoung-Joon
关键词：
过氧化物酶体增殖物激活受体-α (PPARα）调节脂质稳态，特别是在肝脏中。这 这项研究旨在阐明脂肪肝与 禁食期间的氧化应激。禁食PPara基因敲除小鼠 表现出明显的肝脂肪变性，这与高水平的 脂质过氧化、一氧化氮合酶活性和过氧化氢 积累总谷胱甘肽（GSH）、线粒体GSH和 主要的抗氧化酶也较低，在禁食 PPara-无效小鼠。因此， 硝化蛋白在禁食组显著增加， ppara基因敲除小鼠，虽然高水平的蛋白质硝化， 在喂养的Ppara-缺失小鼠中仍然检测到， 氧化修饰的蛋白质只在禁食的 PPara-无效小鼠。然而，炎症在 在禁食的PPara基因敲除小鼠中， 基于肿瘤坏死因子-α的相似水平， 所有群体的变化。这些结果与增加氧化应激观察到， 禁食的PPara-敲除小鼠与其他组相比 证明了PPARα在禁食介导的氧化应激中的作用 抑制PPARα的功能可能会增加 在另一种有毒物质存在下对氧化损伤的敏感性。
The peroxisome proliferator-activated receptor-α (PPARα) regulates lipid homeostasis, particularly in the liver. This study was aimed at elucidating the relationship between hepatosteatosis and oxidative stress during fasting. Fasted Ppara-null mice exhibited marked hepatosteatosis, which was associated with elevated levels of lipid peroxidation, nitric oxide synthase activity, and hydrogen peroxide accumulation. Total glutathione (GSH), mitochondrial GSH, and the activities of major anti-oxidant enzymes were also lower in the fasted Ppara-null mice. Consequently, the number and extent of nitrated proteins were markedly increased in the fasted Ppara-null mice, although high levels of protein nitration were still detected in the fed Ppara-null mice while many oxidatively-modified proteins were only found in the fasted Ppara-null mice. However, the role of inflammation in increased oxidative stress in the fasted Ppara-null mice was minimal based on the similar levels of tumor necrosis factor-α change in all groups. These results with increased oxidative stress observed in the fasted Ppara-null mice compared with other groups demonstrate a role for PPARα in fasting-mediated oxidative stress and that inhibition of PPARα functions may increase the susceptibility to oxidative damage in the presence of another toxic agent.
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影响因子：
4.5
作者：
Gonzalez, Frank J.;Shah, Yatrik A.
通讯作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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