Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen
Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen
复制标题
DOI：
10.1002/anie.201803675
复制
发表时间：
2018-08-13
影响因子：
16.6
通讯作者：
Blacker, A. John
中科院分区：
文献类型：
--
作者：
Chapman, Michael R.;Cosgrove, Sebastian C.;Blacker, A. John
我们报告了一种简单、温和和合成清洁的方法，与传统的间歇式气/液系统相比，可以将酶氧化反应的速度加快高达100倍。生物催化分解双氧水直接在反应介质中产生可溶的O-2源，从而使水中O-2的浓度在安全和实用的条件下增加到超过平衡溶解度。为了更好地利用这种方法，开发了一种新型的流动反应器，以最大限度地提高生产率(g产物L(-1)h(-1))。这种可扩展的台式方法与传统的生物氧化方法相比具有明显的优势，因为它不需要使用加压气体或专门的设备。该方法适用于不同的氧化物酶，并与各种官能团兼容。这些结果最终产生了创纪录的生物氧化时空产率。
We report a simple, mild, and synthetically clean approach to accelerate the rate of enzymatic oxidation reactions by a factor of up to 100 when compared to conventional batch gas/liquid systems. Biocatalytic decomposition of H2O2 is used to produce a soluble source of O-2 directly in reaction media, thereby enabling the concentration of aqueous O-2 to be increased beyond equilibrium solubility under safe and practical conditions. To best exploit this method, a novel flow reactor was developed to maximize productivity (g product L(-1)h(-1)). This scalable benchtop method provides a distinct advantage over conventional bio-oxidation in that no pressurized gas or specialist equipment is employed. The method is general across different oxidase enzymes and compatible with a variety of functional groups. These results culminate in record space-time yields for bio-oxidation.