Serotonin induces selective cleavage of the PKA RI subunit but not RII subunit in Aplysia neurons.
Serotonin induces selective cleavage of the PKA RI subunit but not RII subunit in Aplysia neurons.
复制标题
5-羟色胺诱导海兔神经元中 PKA RI 亚基的选择性裂解，但不诱导 RII 亚基的选择性裂解。
DOI：
10.1016/j.bbrc.2007.05.146
复制
发表时间：
2007
影响因子：
3.1
通讯作者：
Schwartz,JamesH
中科院分区：
文献类型：
--
作者：
Kurosu,Takeshi;Hernández,AIván;Schwartz,JamesH
通过血清素 (5-HT) 刺激，海兔神经元中的 I 型和 II 型 PKA 被激活，从而导致长期促进 (LTF)。调节亚基 (R) 的蛋白水解被认为对于 PKA 的持续激活很重要，而 PKA 是产生 LTF 所必需的。在这项研究中，我们报道了 I 型调节亚基 (RI) 和 II 型调节亚基 (RII) 受到蛋白水解裂解的差异调节。海兔神经元经 5-HT 处理 2 小时后，RI（而非 RII）被选择性裂解。有趣的是，蛋白酶体抑制剂 MG132 抑制了海兔神经元中 5-HT 处理引起的 RI 裂解。此外，用 5-HT 裂解的 35S 标记的 RI 处理的海兔神经节提取物是体外合成的，但不是 35S 标记的 RII。这表明 5-HT 诱导 RI 特异性蛋白水解裂解的激活状态。
PKA type I and type II are activated in Aplysia neurons by stimulation with serotonin (5-HT), which causes long-term facilitation (LTF). The proteolysis of the regulatory subunit (R) is thought important for the persistent activation of PKA, which is necessary to produce LTF. In this study, we report that the type I regulatory subunit (RI) and type II regulatory subunit (RII) are differentially regulated by proteolytic cleavage. RI, but not RII, was selectively cleaved after 5-HT treatment for 2h in Aplysia neurons. Interestingly, the proteasome inhibitor MG132 inhibited the cleavage of RI caused by 5-HT treatment in Aplysia neuron. Besides extracts from Aplysia ganglia treated with 5-HT cleaved35S-labeled RI synthesized in vitro, but not35S-labeled RII. This suggests that 5-HT induces the activation state of RI-specific proteolytic cleavage.