THE ROLE FOR BUTYRATE AND PROPIONATE IN MEDIATING HELA-CELLS GROWTH-INHIBITION BY HUMAN DENTAL PLAQUE FLUID FROM ADULT PERIODONTAL-DISEASE
THE ROLE FOR BUTYRATE AND PROPIONATE IN MEDIATING HELA-CELLS GROWTH-INHIBITION BY HUMAN DENTAL PLAQUE FLUID FROM ADULT PERIODONTAL-DISEASE
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DOI：
10.1016/0003-9969(85)90108-6
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发表时间：
1985-01-01
影响因子：
3
通讯作者：
LEVINE, M
中科院分区：
文献类型：
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作者：
LEVINE, M
为了确定在没有或存在慢性牙周炎的情况下，各种口腔细菌在体外分泌的丙酸和丁酸在多大程度上解释了牙周炎菌斑液体的生长抑制活性，通过用0.13M氯化钠匀浆细菌，离心混合物，并对菌斑液体提取液上清进行微孔过滤灭菌，获得了每种疾病形式的菌斑液体相。通过抑制在添加小牛血清和抗生素的Mini Eagle培养液中生长的[人]HeLa细胞来检测和测量毒素。加入丁酸、丙酸或菌斑液体提取物的样品，测定与对照培养物的生长抑制情况。每种提取物中的毒素都具有热稳定性、冻干性和凝胶过滤洗脱性。丁酸盐和丙酸的含量用惠普5880A型气相色谱仪测定。各组菌斑-液提取物的丁酸含量相似，但大部分活性来源于热敏性高摩尔。WT组件。丁酸和丙酸仅占总活性的5-15%。在患有或不患有牙周炎的受试者的菌斑液中，高分子量外毒素是最突出和最稳定的冻干组织破坏剂，而不是丁酸盐或丙酸。
To determine to what extent propionate and butyrate, secreted in vitro by various oral bacteria, account for the growth-inhibiting activity of plaque fluid in gingivitis in the absence or presence of chronic periodontitis, the fluid phase of plaque from each of these disease forms was obtained by homogenizing the bacteria with 0.13 M NaCl, centrifuging the mixture and Millipore-filter-sterilizing the plaque-fluid-extract supernatant. Toxins were detected and measured by growth inhibition of [human] HeLa cells grown in minimal Eagle medium supplemented with calf serum and antibiotics. Samples of butyrate, propionate or plaque-fluid extract were added and inhibition of growth was determined relative to control cultures. The toxins in each extract were characterized by their stability to heat, freeze-drying and elution on gel filtration. Butyrate and propionate contents were measured with a Hewlett-Packard 5880A gas chromatograph. The plaque-fluid extracts from each group had similar contents of butyrate, but most of the activity was due to heat-sensitive high mol. wt components. Butyrate and propionate accounted for only 5-15% of the total activity. High MW exotoxins, rather than butyrate or propionate, were the most prominent and freeze-drying-stable tissue-destructive agents in the plaque fluid from subjects with or without periodontitis.