The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
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DOI：
10.1105/tpc.013557
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发表时间：
2003-09-01
期刊：
影响因子：
11.6
通讯作者：
Chua, NH
中科院分区：
文献类型：
--
作者：
Hu, YX;Xie, O;Chua, NH
在植物发育过程中，器官的最终大小受各种发育信号的调节和决定;然而，这些信号转导的分子机制和所涉及的介质在很大程度上是未知的。在这里，我们表明，ARGOS，一个新的拟南芥基因，是高度诱导生长素，参与器官大小的控制。表达正义或反义ARGOS cDNA的转基因植物分别显示出增大或缩小的地上器官。器官大小的改变主要归因于细胞数量和器官生长持续时间的变化。ARGOS的异位表达与AINTEGUMENTA（ANT）和CycD 3;1的表达以及叶细胞的肿瘤活性相关。此外，在过表达ARGOS的植物中，ANT功能的丧失可以阻止器官的扩大，这意味着ARGOS在ANT的上游发挥作用，影响器官细胞的分生能力。生长素对ARGOS的诱导在生长素抗性1（axr 1）中减弱或消除，并且ARGOS的过表达部分恢复axr 1器官发育。这些结果表明，在器官发生过程中，ARGOS可能将生长素信号传导至AXR 1下游，通过ANT调控细胞增殖和器官生长。
During plant development, the final size of an organ is regulated and determined by various developmental signals; however, the molecular mechanisms by which these signals are transduced and the mediators involved are largely unknown. Here, we show that ARGOS, a novel Arabidopsis gene that is highly induced by auxin, is involved in organ size control. Transgenic plants expressing sense or antisense ARGOS cDNA display enlarged or reduced aerial organs, respectively. The alteration in organ size is attributable mainly to changes in cell number and the duration of organ growth. Ectopic expression of ARGOS prolongs the expression of AINTEGUMENTA (ANT) and CycD3;1 as well as the neoplastic activity of leaf cells. Moreover, organ enlargement in plants over-expressing ARGOS can be blocked by the loss of function of ANT, implying that ARGOS functions upstream of ANT to affect the meristematic competence of organ cells. The induction of ARGOS by auxin is attenuated or abolished in auxin-resistant1 (axr1), and overexpression of ARGOS partially restores axr1 organ development. These results suggest that ARGOS may transduce auxin signals downstream of AXR1 to regulate cell proliferation and organ growth through ANT during organogenesis.