EXPRESSION OF EARLY GENES OF ORIGIN-DEFECTIVE MUTANTS OF SIMIAN-VIRUS-40
EXPRESSION OF EARLY GENES OF ORIGIN-DEFECTIVE MUTANTS OF SIMIAN-VIRUS-40
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DOI：
10.1073/pnas.77.7.3898
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
FRISQUE, RJ
中科院分区：
文献类型：
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作者：
GLUZMAN, Y;SAMBROOK, JF;FRISQUE, RJ
测定了8个SV 40起源缺陷突变株的核苷酸序列。所有突变体都遭受了缺失，其大小范围为4-241个核苷酸。一些突变体诱导肿瘤（T）抗原的合成，另一些则不诱导。用Berk和Sharp的S1核酸酶技术分析了从由2个T[肿瘤]抗原阳性突变体转化的大鼠细胞中提取的病毒mRNA。无论缺失的大小或位置如何，病毒mRNA的5“末端位于距富含A + T的区域（A-T-T-T-A-T）大致相同的距离处，而不是位于病毒基因组中的特定位点处。
The nucleotide sequences of 8 origin-defective mutants of SV40 were determined. All of the mutants suffered deletions, which range in size from 4-241 nucleotides. Some of the mutants induce the synthesis of tumor (T) antigen, others do not. Viral mRNA extracted from rat cells transformed by 2 of the T[tumor]-antigen-positive mutants was analyzed by the S1 nuclease technique of Berk and Sharp. Irrespective of the size or the location of the deletions, the 5'' ends of viral mRNA are located approximately the same distance from the A + T-rich region (A-T-T-T-A-T) rather than at a specific site in the viral genome.