Roles of neuraminidase in the initial stage of influenza virus infection
Roles of neuraminidase in the initial stage of influenza virus infection
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DOI：
10.1016/j.micinf.2005.12.008
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发表时间：
2006-04-01
影响因子：
5.8
通讯作者：
Ohuchi, Reiko
中科院分区：
文献类型：
--
作者：
Ohuchi, Masanobu;Asaoka, Naoko;Ohuchi, Reiko
我们提出了一个概念，即神经氨酸酶（NA）促进病毒进入靶细胞在病毒感染的初始阶段，除了普遍接受的概念，流感病毒NA促进子代病毒从宿主细胞中释放在病毒复制的最后阶段。当用特异性抑制剂如扎那米韦和奥司他韦羧酸盐抑制NA活性时，病毒对MDCK和A549细胞的感染效率分别降低至约1/4和1/8。NA抑制剂没有显着影响病毒结合和包膜融合活动，当使用红细胞和病毒系统进行评估。由于病毒感染的初始阶段涉及病毒与靶细胞的结合、病毒进入内体和包膜与内体膜的融合，我们的结果表明NA抑制剂干扰病毒进入步骤。因此，NA被认为促进病毒进入，从而提高感染效率。(c)2006年，Elsevier SAS。All rights reserved.
We propose a concept that neuraminidase (NA) promotes virus entry into target cells during the initial stage of viral infection, in addition to the generally accepted concept that influenza virus NA promotes the release of progeny virus from a host cell at the final stage of viral replication. When NA activity was inhibited with specific inhibitors such as zanamivir and oseltamivir carboxylate, infection efficiency of the virus to MDCK and A549 cells was reduced to approximately 1/4 and 1/8, respectively. NA inhibitors did not significantly affect virus binding and envelope fusion activities, when assessed using an erythrocyte and virus system. Since the initial stage of viral infection involves binding of the virus to the target cell, virus entry into an endosome and envelope fusion with the endosomal membrane, our results indicated that NA inhibitors interfered with the virus entry step. Thus, NA is thought to promote virus entry, and thereby enhances infection efficiency. (c) 2006 Elsevier SAS. All rights reserved.