Collagen density significantly affects the functional properties of an engineered provisional scaffold
Collagen density significantly affects the functional properties of an engineered provisional scaffold
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DOI：
10.1002/jbm.a.32508
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发表时间：
2010-04-01
影响因子：
4.9
通讯作者：
Murray, Martha M.
中科院分区：
文献类型：
--
作者：
Abreu, Eduardo L.;Palmer, Matthew P.;Murray, Martha M.
临时支架的形成对于伤口愈合至关重要。然而，对于关节内部的组织来说，这一过程受到滑液环境的阻碍，从而导致伤口愈合明显受损。因此，开发在关节内环境中有效的替代临时支架引起了人们的极大兴趣。最近发现胶原蛋白-血小板水凝胶可用作替代临时支架材料。在这项研究中，我们的假设是，增加水凝胶中的胶原蛋白密度会导致生理变化，这可能会影响它们作为临时支架替代品的功能。主要功能结果测量是水凝胶模量、血小板活化、成纤维细胞增殖和支架回缩。胶原密度的增加导致胶原-血小板水凝胶具有更高的储能模量。在测试范围内，未发现血小板活化依赖于胶原蛋白密度。增加胶原密度对成纤维细胞增殖和支架回缩具有抑制作用。这些研究表明，胶原蛋白密度可能能够显着影响用作替代临时支架的胶原蛋白-血小板水凝胶的功能。 (C) 2009 Wiley periodicals, Inc. J Biomed Mater Res 93A: 150-157, 2010
The formation of a provisional scaffold is essential in wound healing. However, for tissues inside of joints, this process is impeded by the synovial fluid environment and wound healing is significantly impaired as a result. Therefore, development of substitute provisional scaffolds which are effective in the intra-articular environment is of great interest. Collagen-platelet hydrogels have recently been found useful as substitute provisional scaffolding materials. In this study, our hypothesis was that increasing the collagen density in the hydrogel Would result in physiologic changes that would be likely to affect their function as provisional scaffold substitutes. The primary functional outcome measures were modulus of the hydrogel, platelet activation, fibroblast proliferation, and scaffold retraction. Increased collagen density resulted in collagen-platelet hydrogels with a higher storage modulus. Platelet activation was not found to be dependent on the collagen density within the range tested. Increasing the collagen density had a suppressive effect on both fibroblast proliferation and scaffold retraction. These studies suggest that the collagen density may be able to significantly influence the function of collagen-platelet hydrogels used as substitute provisional scaffolds. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 150-157, 2010