Anaerobic ammonium oxidation in sediments of surface flow constructed wetlands treating swine wastewater
Anaerobic ammonium oxidation in sediments of surface flow constructed wetlands treating swine wastewater
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处理养猪废水的地表流人工湿地沉积物中的厌氧氨氧化
DOI：
10.1007/s00253-016-7957-y
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发表时间：
2017-02-01
影响因子：
5
通讯作者：
Wu, Jin-shui
中科院分区：
文献类型：
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作者：
Chen, Liang;Liu, Feng;Wu, Jin-shui
厌氧氨氧化（anammox）被认为参与了人工湿地（CWs）脱氮过程。然而，它的发生和在处理猪场废水的CWs的作用还没有得到很好的评价。在这项研究中，我们调查了厌氧氨氧化细菌的多样性，活性和中尺度表面流CW（SFCWs）的沉积物中的作用进行了不同的氮负荷的猪废水。我们发现厌氧氨氧化细菌在SFCW沉积物中很丰富，每克干土中标记hzsB基因的拷贝数为7.5 × 10（5）至3.5 × 10（6）。稳定同位素示踪结果表明，潜在厌氧氨氧化速率为1.03 ~ 12.5nmolNg（-1）·h（-1），占总N-2产量的8.63-57.1%。厌氧氨氧化过程对氮的总去除率为0.83-2.68 kg N·年（-1），约为0.85 kg N·年（-1）。N负载的10%。16 S核糖体RNA（rRNA）的系统发育分析显示，存在多个共存的anammox属，包括“Bracidatus Brocadia”作为最常见的一个，“Ca. Kuenenia，”“Ca. Scalindua，”和四个新的身份不明的集群。相关性分析表明，厌氧氨氧化菌的活性和丰度与沉积物pH、NH 4（+）-N和NO2（-）-N密切相关。总之，我们的研究结果证实了不同的厌氧氨氧化细菌的存在，并表明厌氧氨氧化过程可以作为一个有前途的脱氮途径在处理猪废水的SFCWs。
Anaerobic ammonium oxidation (anammox) was suggested to be involved in the nitrogen (N) removal process in constructed wetlands (CWs). Nevertheless, its occurrence and role in CWs treating swine wastewater have not been well evaluated yet. In this study, we investigated the diversity, activity, and role of anammox bacteria in sediments of mesoscale surface flow CWs (SFCWs) subjected to different N loads of swine wastewater. We found that anammox bacteria were abundant in SFCW sediments, as indicated by 7.5 x 10(5) to 3.5 x 10(6) copies of the marker hzsB gene per gram of dry soil. Based on stable isotope tracing, potential anammox rates ranged from 1.03 to 12.5 nmol N g(-1) dry soil h(-1), accounting for 8.63-57.1% of total N-2 production. We estimated that a total N removal rate of 0.83-2.68 kg N year(-1) was linked to the anammox process, representing ca. 10% of the N load. Phylogenetic analyses of 16S ribosomal RNA (rRNA) revealed the presence of multiple co-occurring anammox genera, including "Candidatus Brocadia" as the most common one, "Ca. Kuenenia," "Ca. Scalindua," and four novel unidentified clusters. Correlation analyses suggested that the activity and abundance of anammox bacteria were strongly related to sediments pH, NH4 (+)-N, and NO2 (-)-N. In conclusion, our results confirmed the presence of diverse anammox bacteria and indicated that the anammox process could serve as a promising N removal pathway in the treatment of swine wastewater by SFCWs.