Top-down mass spectrometry on low-resolution instruments: Characterization of phosphopantetheinylated carrier domains in polyketide and non-ribosomal biosynthetic pathways
Top-down mass spectrometry on low-resolution instruments: Characterization of phosphopantetheinylated carrier domains in polyketide and non-ribosomal biosynthetic pathways
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DOI：
10.1016/j.bmcl.2007.10.104
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发表时间：
2008-05-15
影响因子：
2.7
通讯作者：
Dorrestein, Pieter C.
中科院分区：
文献类型：
--
作者：
Meluzzi, Dario;Zheng, Wei Hao;Dorrestein, Pieter C.
质谱 (MS) 是研究非核糖体肽、聚酮化合物和脂肪酸生物合成的重要工具。在这里，我们描述了一种在普通离子阱仪器上使用多级串联 MS 的新方法，以获得与磷酸泛酰乙胺化 (holo) 载体蛋白结合的底物和中间体质量的高分辨率测量。特别是，我们报告了在 MS(2) 过程中从全息载体蛋白中喷射出的磷酸泛硫氨酸部分的 12 个诊断性 MS(3) 片段的化学式。我们通过观察 Holo-AcpC（一种来自无乳链球菌的假定酰基载体蛋白）的形成来证明我们的方法。由爱思唯尔有限公司出版
Mass spectrometry (MS) is an important tool for studying non-ribosomal peptide, polyketide, and fatty acid biosynthesis. Here we describe a new approach using multi-stage tandem MS on a common ion trap instrument to obtain high-resolution measurements of the masses of substrates and intermediates bound to phosphopantetheinylated ( holo) carrier proteins. In particular, we report the chemical formulas of 12 diagnostic MS(3) fragments of the phosphopantetheine moiety ejected from holo carrier proteins during MS(2). We demonstrate our method by observing the formation of holo-AcpC, a putative acyl carrier protein from Streptococcus agalactiae. Published by Elsevier Ltd.