Controlled basic fibroblast growth factor release device made of poly(ethyleneglycol) dimethacrylates for creating a subcutaneous neovascular bed for cell transplantation
Controlled basic fibroblast growth factor release device made of poly(ethyleneglycol) dimethacrylates for creating a subcutaneous neovascular bed for cell transplantation
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由聚乙二醇二甲基丙烯酸酯制成的受控碱性成纤维细胞生长因子释放装置，用于创建用于细胞移植的皮下新生血管床
DOI：
10.1002/jbm.a.36153
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Abe Toshiaki
中科院分区：
文献类型：
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作者：
Yamada Shinji;Nagai Nobuhiro;Saijo Saaya;Kaji Hirokazu;Nishizawa Matsuhiko;Imura Kozue;Goto Masafumi;Abe Toshiaki
皮下间隙是胰岛等细胞移植治疗1型糖尿病的潜在部位。为了促进移植，需要为移植细胞提供最佳的生长空间和新生血管的形成。在这项研究中，我们开发了一种使用光固化树脂聚乙二醇二甲基丙烯酸酯（PEGDM）的装置，用于控制碱性成纤维细胞生长因子（bFGF）的释放，以在大鼠中创建皮下新血管床。该装置由带有微孔的圆盘状胶囊组成，由三（乙二醇）二甲基丙烯酸酯（TEGDM）和PEGDM药物制剂组成。通过改变药物制剂中孔隙的数量以及水和PEGDM的组成来调节药物的释放速度。从磷酸盐缓冲盐水（PBS）中培养的装置中释放的bFGF促进了成纤维细胞的生长，表明bFGF释放后的生物活性。组织学评估显示血管范围的显著增加，这取决于装载到装置中的bFGF的量。用异硫氰酸葡聚糖2000 kDa荧光素进行灌注研究显示线性和毛细血管染色模式，表明血管功能强大。综上所述，可控bFGF释放装置可在皮下空间提供具有所需血管化的新生血管床。©2017 Wiley期刊公司[J] .中国生物医学工程学报，2016,35(5):557 - 557。
Subcutaneous space is a potential site for the transplantation of cells such as islets for treatment of type 1 diabetes. To enhance engraftment, an optimal space for the growth of the transplanted cells is needed along with neovascularization. In this study, we developed a device using a photocurable resin, poly(ethyleneglycol) dimethacrylates (PEGDM), for controlled release of basic fibroblast growth factor (bFGF) to create a subcutaneous neovascular bed in rats. The device consists of a disk‐shaped capsule with micropores and is composed of tri(ethyleneglycol) dimethacrylate (TEGDM) and a drug formulation of PEGDM. The release rate was tuned by changing the number of pores and the composition of water and PEGDM in the drug formulation. bFGF released from devices incubated in phosphate‐buffered saline (PBS) enhanced the growth of fibroblasts, indicating bioactivity of bFGF after release. Histological evaluation showed a significant increase in the extent of vasculature that was dependent on the amount of bFGF loaded into the device. A perfusion study using fluorescein isothiocyanate dextran 2000 kDa showed linear and capillary staining patterns, indicating potent functional vasculature. In conclusion, the controlled bFGF releasing device could provide a neovascular bed with the required vascularization in the subcutaneous space. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3017–3024, 2017.