Mitochondrial NADH oxidase activity of adult Hymenolepis diminuta (Cestoda).

Mitochondrial NADH oxidase activity of adult Hymenolepis diminuta (Cestoda).
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成年 Hymenolepis diminuta (Cestoda) 的线粒体 NADH 氧化酶活性。

DOI:
10.1016/0305-0491(82)90511-9
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发表时间:
1982
期刊:
Comparative biochemistry and physiology. B, Comparative biochemistry
影响因子:
--
通讯作者:
Fioravanti,CF
Fioravanti,CF
中科院分区:
--
文献类型:
--
作者:
Fioravanti,CF

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1.从成年Hymenolepis diminuta线粒体显示膜相关的,鱼藤酮敏感的NADH氧化酶和NADH依赖的延胡索酸还原酶系统。2. H.缺陷氧化酶和延胡索酸还原酶对抗霉素A相对不敏感。氰化钾和叠氮化钠,在显着抑制大鼠肝脏的NADH氧化酶系统的浓度。3.丙二酸能有效抑制H. diminuta和猪蛔虫成虫(线虫纲)。4. Mn ~(2+)参与了H. diminuta氧化酶,是明显的。5.利用NAD(P)H,H. diminuta,线粒体膜导致过氧化氢的形成。琥珀酸盐的利用也导致过氧化物的积累,但其速度比NAD(P)H慢得多。
1. Mitochondria from adult Hymenolepis diminuta displayed a membrane-associated, rotenone-sensitive NADH oxidase and NADH-dependent fumarate reductase system. 2. Both the H. diminuta oxidase and fumarate reductase were relatively insensitive to antimycin A. potassium cyanide and sodium azide, at concentrations which significantly inhibited the NADH oxidase system of rat liver. 3. Malonate effectively depressed the mitochondrial NADH oxidase activity of both H. diminuta and adult Ascaris suum (Nematoda). 4. An involvement of Mn2+ ion, in NADH utilization by the H. diminuta oxidase, was apparent. 5. The utilization of NAD (P) H by H. diminuta, mitochondrial membranes resulted in hydrogen peroxide formation. Succinate utilization also resulted in peroxide accumulation but at a much slower rate than that found for NAD (P) H.
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