Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues.
Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues.
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发表时间：
1986-11
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通讯作者：
I. Damjanov;B. Mildner;B. Knowles
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作者：
I. Damjanov;B. Mildner;B. Knowles
一种识别人表皮生长因子（EGF）受体外结构域表位的单克隆抗体被用于在选定的正常人体组织中定位该蛋白。两种类型的反应性被识别：强线状或颗粒状细胞表面染色和颗粒状细胞质染色。在一个组织，子宫内膜中，观察到与激素刺激变化相关的反应模式的变化。在一些组织中，如附睾和皮肤，抗体表现出表面反应性，被认为是代表部分增殖细胞室的细胞，而在肝脏、胰腺和前列腺，所有细胞都与抗体反应，尽管主要反应性局限于细胞质。表皮生长因子受体对特定细胞类型和细胞区室的差异分布可能表明适应允许生长因子在可用配体的环境中产生反应。
A monoclonal antibody recognizing an epitope of the external domain of the human epidermal growth factor (EGF) receptor was used to localize this protein in selected normal human tissues. Two patterns of reactivity were recognized: strong linear or granular cell surface staining, and granular cytoplasmic staining. In one tissue, the endometrium, a change in the reaction pattern associated with changes in hormonal stimulation was observed. In some tissues such as epididymis and skin, the antibody showed surface reactivity with cells considered to represent part of the proliferating cell compartment, whereas in liver, pancreas, and prostate, all cells were reactive with the antibody, though the predominant reactivity was localized in the cytoplasm. The differential distribution of the epidermal growth factor receptor to specific cell types and cellular compartments may signify adaptations that permit growth factor responsiveness in a milieu of available ligand.