Expression of an X-family DNA polymerase, pol lambda, in the respiratory epithelium of non-small cell lung cancer patients with habitual smoking
Expression of an X-family DNA polymerase, pol lambda, in the respiratory epithelium of non-small cell lung cancer patients with habitual smoking
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DOI：
10.1016/j.mrgentox.2009.05.008
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发表时间：
2009-06-01
影响因子：
1.9
通讯作者：
Maehara, Yoshihiko
中科院分区：
文献类型：
--
作者：
Ohba, Taro;Kometani, Takurou;Maehara, Yoshihiko
DNA聚合酶λ（pol λ）是X家族DNA聚合酶的真核成员，其参与两种DNA修复模式，即碱基切除修复（BER）或非同源末端连接（NHEJ）。使用免疫组织化学方法，我们观察到pol λ在人体组织中的表达，特别是在肺癌患者的呼吸系统中。pol λ蛋白分布在支气管、细支气管和肺泡中的上皮细胞的细胞核中。有趣的是，支气管上皮细胞中pol λ的表达水平与个体中习惯性吸烟的量显著相关。相反，癌组织中的pol λ表达与患者的吸烟状况无关。Pol λ的表达有时在肿瘤组织和邻近细支气管之间存在差异。更重要的是，在重度吸烟者中发现的没有pol λ表达的肿瘤明显倾向于处于晚期临床阶段。因此，Pol lambda可能参与DNA修复过程，抵消烟草烟雾在呼吸系统中引起的DNA损伤。(C)2009爱思唯尔有限公司版权所有。
DNA polymerase lambda, pol lambda, is a eukaryotic member of the X-family DNA polymerases that is involved in two modes of DNA repair, i.e. base excision repair (BER) or non-homologous end joining (NHEJ). Using immunohistochemical approaches, we have observed pol lambda expression in human tissues, particularly in the respiratory system of lung cancer patients. pol lambda proteins were distributed in the nuclei of the epithelial cells in the bronchi, bronchioles and alveoli. Intriguingly, the level of pol lambda expression in the bronchiolar epithelia significantly correlated with the amount Of habitual smoking in the individuals. Conversely, pol lambda expression in cancer tissues did not correlate with the smoking status of the patients. Pol lambda expression was sometimes discrepant between the tumor tissues and adjacent bronchioles. More importantly, tumors without pol lambda expression that Occurred in heavy smokers significantly tended to be at an advanced clinical stage. Pol lambda may thus be involved in the DNA repair Processes Counteracting DNA damage caused by tobacco smoke in the respiratory system. (C) 2009 Elsevier B.V. All rights reserved.