Differential contractile responsiveness of femoral arteries from healthy and diabetic dogs: role of endothelium.
Differential contractile responsiveness of femoral arteries from healthy and diabetic dogs: role of endothelium.
复制标题
健康狗和糖尿病狗股动脉的不同收缩反应：内皮的作用。
DOI：
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发表时间：
1987
影响因子：
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通讯作者：
P. Hadházy
中科院分区：
文献类型：
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作者：
D. Gebremedhin;M. Koltai;G. Pogátsa;K. Magyar;P. Hadházy
测定糖尿病（四氧嘧啶治疗）和代谢健康犬的离体股动脉条（有和无内皮）的苯肾上腺素诱导的收缩和乙酰胆碱诱导的舒张。四氧嘧啶处理不改变内皮完整的动脉对苯肾上腺素（PE）的收缩反应性。然而，在机械去除内皮层后，糖尿病血管产生的最大力（22.0 +/- 2.0 mN.m-2）显著超过非糖尿病动脉产生的最大收缩（14.6 +/- 1.8 mN.m-2）。糖尿病动脉对PE的剂量反应曲线比非糖尿病动脉条更陡。两组PE的EC 50值相似（糖尿病和非糖尿病血管分别为0.45 +/- 0.12和0.58 +/- 0.20 mumol/l）。在内皮完整的动脉中，乙酰胆碱产生浓度相关的PE诱导的张力降低。乙酰胆碱的这种内皮依赖性舒张活性在健康和糖尿病动脉条中相似，乙酰胆碱的IC 50分别为0.17 +/- 0.02和0.20 +/- 0.03 mumol/l。这些结果表明，内皮功能改变（可能是EDRF释放增加）在糖尿病中普遍存在。这在降低糖尿病动脉平滑肌对PE的高反应性方面可能是重要的。
Phenylephrine-induced contraction and acetylcholine-induced relaxation of isolated femoral arterial strips (with and without endothelium) of diabetic (alloxan-treated) and metabolically healthy dogs were determined. Alloxan treatment did not change the contractile responsiveness to phenylephrine (PE) of the arteries with intact endothelium. After mechanical removal of the endothelial layer, however, the maximum force generated by the diabetic vessels (22.0 +/- 2.0 mN.m-2) significantly exceeded the maximum contraction produced by the nondiabetic arteries (14.6 +/- 1.8 mN.m-2). The dose-response curve of diabetic arteries to PE was steeper than it was in non-diabetic strips. The EC50 values for PE were similar in these two groups (0.45 +/- 0.12 and 0.58 +/- 0.20 mumol/l in diabetic and nondiabetic vessels, respectively). In the arteries with intact endothelium, acetylcholine produced concentration-related reduction of PE-induced tone. This endothelium-dependent relaxant activity of acetylcholine was similar in the healthy and diabetic arterial strips, IC50 for acetylcholine being 0.17 +/- 0.02 and 0.20 +/- 0.03 mumol/l, respectively. These results suggest that functional alteration of endothelium (probably an increased release of EDRF) prevails in diabetes. This may be important in reducing the hyper-responsiveness of diabetic arterial smooth muscle to PE.