Differential stimulation of microglial pro-inflammatory cytokines by Acanthamoeba culbertsoni versus Acanthamoeba castellanii.
Differential stimulation of microglial pro-inflammatory cytokines by Acanthamoeba culbertsoni versus Acanthamoeba castellanii.
复制标题
库伯氏棘阿米巴与卡氏棘阿米巴对小胶质细胞促炎细胞因子的不同刺激。
DOI：
10.1111/j.1550-7408.2004.tb00398.x
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Cabral,GuyA
中科院分区：
文献类型：
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作者：
Marciano-Cabral,Francine;Ludwick,Christina;Puffenbarger,RobynA;Cabral,GuyA
Acanthamoebaspp. are opportunistic pathogens that cause granulomatous amebic encephalitis. We compared the highly pathogenic speciesA. culbertsonito the relatively less pathogenic speciesA. castellaniifor its capacity to elicit from neonatal rat microglia the gene expression of pro‐inflammatory cytokines.Acanthamoeba culbertsonielicited a robust cytokine gene response by neonatal rat microglia in vitro as compared toA. castellanii. The preponderant cytokine elicited at the mRNA and protein levels was interleukin‐1β. In addition, transmission electron microscopy revealed that microglial cells were capable of phagocytozingA. castellanii. In contrast,A. culbertsonidestroyed microglia. Collectively, these results suggest that a combined action of pro‐inflammatory cytokines and destruction of host cells by amebae contribute to the pathology caused by the more pathogenic species.
