Immunocytochemical localization of Na+, K+-ATPase in the canine choroid plexus.
Immunocytochemical localization of Na+, K+-ATPase in the canine choroid plexus.
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DOI：
10.1093/brain/108.3.625
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发表时间：
1985-09
期刊：
影响因子：
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通讯作者：
T. Masuzawa;T. Ohta;K. Kawakami;F. Sato
中科院分区：
文献类型：
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作者：
T. Masuzawa;T. Ohta;K. Kawakami;F. Sato
为了确定犬第四脑室脉络丛中 Na+、K+-ATP 酶的定位，使用了犬肾 Na+、K+-ATP 酶全酶的抗血清以及该酶的 α 和 β 亚基，并通过过氧化物酶-抗过氧化物酶免疫组织化学在光和电子显微镜水平上检查反应产物的分布。 方法。在脉络膜上皮细胞中，抗原位点仅限于顶端质膜的外表面，包括微绒毛的膜。在细胞质中或沿侧质膜和基底质膜未检测到反应产物。这些发现强化了这样的假设：由于 Na+、K+-ATP 酶介导的主动转运，脉络膜上皮细胞的顶缘在脑脊液分泌中发挥着重要作用。
To determine the localization of Na+, K+-ATPase in the choroid plexus of the canine fourth ventricle, antisera against canine renal Na+, K+-ATPase holoenzyme and the alpha and beta subunits of the enzyme were employed and the distribution of reaction product examined at the light and electron microscopic level by means of the peroxidase-antiperoxidase immunohistochemical method. In the choroidal epithelial cells, antigenic sites were restricted to the outer surface of the apical plasmalemma, including the membranes of the microvilli. No reaction product was detected in the cytoplasm or along the lateral and basal plasmalemma. These findings strengthen the hypothesis that the apical border of the choroidal epithelial cell plays an important role in cerebrospinal fluid secretion as a result of active transport mediated by Na+, K+-ATPase.