Systemic mononuclear-cell vasculitis in MRL/Mp-lpr/lpr mice. A histologic and immunocytochemical analysis.
Systemic mononuclear-cell vasculitis in MRL/Mp-lpr/lpr mice. A histologic and immunocytochemical analysis.
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发表时间：
1987-05
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通讯作者：
C. Moyer;J. Strandberg;C. Reinisch
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作者：
C. Moyer;J. Strandberg;C. Reinisch
单核细胞血管炎表达的细胞机制知之甚少。为了确定自身免疫性MRL/lpr小鼠中血管炎发生的事件的精确顺序，用一组细胞化学和免疫组织化学染色评价了4-20周龄小鼠的组织切片。结果显示，MRL/lpr小鼠的血管疾病发展如下：Thy 1+、Ly 1+、L3 T4- T细胞在约8周龄时主要聚集在中小肌动脉周围。在12周龄时，外膜炎性病灶形成，由邻近肥大血管平滑肌细胞（VSMC）的大的“反应性”单核炎性细胞组成。原始Thy 1+、Ly 1+、L3 T4- T细胞随后浸润图尼卡中膜，并导致选择性VSMC核溶解。偶尔观察到细胞毒性/抑制性T细胞、巨噬细胞和可能的NK细胞，主要位于浸润部位远端。炎性浸润的外区由成熟的B细胞和偶尔的B细胞前体组成。这些结果表明，细胞成分的免疫反应介导单核细胞血管炎的MRL/lpr小鼠。
The cellular mechanisms governing the expression of mononuclear cell vasculitis are poorly understood. For determination of the precise sequence of events in the development of vasculitis in autoimmune MRL/lpr mice, histologic sections from 4-20-week-old mice were evaluated with a panel of cytochemical and immunohistochemical stains. The results show that vascular disease in MRL/lpr mice develops as follows: Thy 1+, Ly 1+, L3T4- T cells assemble around predominantly small-to-medium muscular arteries at approximately 8 weeks of age. At 12 weeks of age, an adventitial inflammatory focus forms, composed of large "reactive" mononuclear inflammatory cells adjacent to hypertrophied vascular smooth muscle cells (VSMCs). Blastic Thy 1+, Ly 1+, L3T4- T cells subsequently infiltrate the tunica media, and selective VSMC karyolysis results. Occasional cytotoxic/suppressor T cells, macrophages, and possibly NK cells are noted primarily distal to the infiltration site. The outer zone of the inflammatory infiltrate is composed of mature B cells and occasional B-cell precursors. These findings suggest that cellular constituents of the immune response mediate mononuclear cell vasculitis in MRL/lpr mice.