Prostaglandin F2alpha induces expression of prostaglandin G/H synthase-2 in the ovine corpus luteum: a potential positive feedback loop during luteolysis.
Prostaglandin F2alpha induces expression of prostaglandin G/H synthase-2 in the ovine corpus luteum: a potential positive feedback loop during luteolysis.
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前列腺素 F2α 诱导绵羊黄体中前列腺素 G/H 合酶 2 的表达：黄体溶解过程中的潜在正反馈回路。
DOI：
10.1095/biolreprod57.5.1016
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发表时间：
1997
影响因子：
3.6
通讯作者：
Wiltbank,MC
中科院分区：
文献类型：
--
作者：
Tsai,SJ;Wiltbank,MC
前列腺素（PG）F2 α在黄体退化中的主要作用已在许多哺乳动物物种中得到明确证实。我们采用体内和体外方法研究了外源性PGF 2 α诱导绵羊黄体细胞表达前列腺素G/H合酶-2（PGHS-2;环氧合酶-2）并导致PGF 2 α产生的可能性。将1 ml PGF 2 α（1 μ mol）或生理盐水注入卵巢动脉，在不同时间收集黄体，采用定量竞争性逆转录聚合酶链反应分析PGHS-2 mRNA。PGF 2 α。显著增加PGHS-2 mRNA的稳态浓度在1小时内，但基础浓度在处理后12小时恢复。使用分离的绵羊大黄体细胞进行的体外研究表明，PGHS-2的mRNA被PGF 2、佛波醇双癸酸酯和离子霉素以与体内观察到的模式相似的模式诱导。3种处理均诱导PGHS-2蛋白表达，处理后~h，PGF 2 α积累。在处理后12和24 h，培养基中的浓度增加。提示PGF_（2 α）可能通过蛋白激酶C/胞内游离钙通道刺激大黄体细胞表达PGHS-2并产生PGF_（2 α）。这种黄体PGF 2 α可能具有自分泌/旁分泌功能，以增强子宫来源的PGF 2 α的溶黄体作用。
The primary role of prostaglandin (PG) F2αin regression of the corpus luteum has been clearly demonstrated in many mammalian species. We have used in vivo and in vitro approaches to investigate the possibility that exogenous PGF2αinduces expression of prostaglandin G/H synthase-2 (PGHS-2; cyclooxygenase- 2) and causes production of PGF2αin ovine luteal cells. Ewes received infusions into the ovarian artery of 1 ml PGF2α(1 μmol) or saline, and corpora lutea were collected at various times and analyzed for PGHS-2 mRNA using quantitative, competitive reverse transcription polymerase chain reaction. PGF2α. dramatically increased the steady-state concentration of mRNA for PGHS-2 within 1 h, but basal concentration returned at 12 h posttreatment. In vitro studies using isolated ovine large luteal cells indicated that mRNA for PGHS-2 was induced by PGF2α'phorbol didecanoate, and ionomycin in a pattern similar to that observed in vivo. PGHS-2 protein was induced by all three treatments – h later, and accumulation of PGF2α. in the culture media increased at 12 and 24 h posttreatment. In conclusion, we have provided evidence that PGF2α' probably acting through the protein kinase C/free intracellular calcium pathway, can stimulate large luteal cells to express PGHS-2 and produce PGF2α. This luteal PGF2αis likely to have an autocrine/paracrine function to augment the luteolytic effect of PGF2αof uterine origin.