Chronic intermittent hypoxia alters ventilatory and metabolic responses to acute hypoxia in rats.
Chronic intermittent hypoxia alters ventilatory and metabolic responses to acute hypoxia in rats.
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慢性间歇性缺氧改变了大鼠对急性缺氧的通气和代谢反应。
DOI：
10.1152/japplphysiol.00015.2016
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Dopp,JohnM
中科院分区：
文献类型：
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作者：
Morgan,BarbaraJ;Adrian,Russell;Wang,Zun-Yi;Bates,MelissaL;Dopp,JohnM
我们确定了慢性暴露于间歇性缺氧（CIH）对清醒动物通气的化学反射控制的影响。将成年雄性Sprague-Dawley大鼠暴露于CIH [最低血氧饱和度（SpO 2），75%; 15次事件/h; 10 h/天]或常氧（NORM）21天。我们评估了暴露前后对急性分级缺氧的以下反应：通气（V ~ e，通过气压体积描记法）、V ~ o2和V ~ co2（分析呼出气体）、心率（HR）和SpO 2（通过颈领脉搏血氧测定法）。我们通过计算SpO 2和V ~（tao）co2的等效浓度之间的刺激-反应关系（线性回归）来量化低氧诱导的化学感受器敏感性。另一个目的是确定CIH是否导致颈动脉体球细胞增殖（使用溴脱氧尿苷）。CIH暴露增加了V_e/V_tao_2/SpO_2关系的斜率，并在常氧下引起过度通气。CIH和NORM的溴脱氧尿苷染色相当。因此，我们的CIH范例增加缺氧化疗敏感性，而不引起血管球细胞增殖。
We determined the effects of chronic exposure to intermittent hypoxia (CIH) on chemoreflex control of ventilation in conscious animals. Adult male Sprague-Dawley rats were exposed to CIH [nadir oxygen saturation (SpO2), 75%; 15 events/h; 10 h/day] or normoxia (NORM) for 21 days. We assessed the following responses to acute, graded hypoxia before and after exposures: ventilation (V̇e, via barometric plethysmography), V̇o2and V̇co2(analysis of expired air), heart rate (HR), and SpO2(pulse oximetry via neck collar). We quantified hypoxia-induced chemoreceptor sensitivity by calculating the stimulus-response relationship between SpO2and the ventilatory equivalent for V̇co2(linear regression). An additional aim was to determine whether CIH causes proliferation of carotid body glomus cells (using bromodeoxyuridine). CIH exposure increased the slope of the V̇e/V̇co2/SpO2relationship and caused hyperventilation in normoxia. Bromodeoxyuridine staining was comparable in CIH and NORM. Thus our CIH paradigm augmented hypoxic chemosensitivity without causing glomus cell proliferation.